Linked Life Data

9325278

Source:http://linkedlifedata.com/resource/pubmed/id/9325278

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
41
pubmed:dateCreated
1997-11-13
pubmed:databankReference
pubmed:abstractText
We have utilized the polymerase chain reaction (PCR)-based differential display methodology (Liang, P., and Pardee, A. B. (1992) Science 257, 967-969) to identify a novel transcript whose expression levels increased in Xenopus embryo explants during mesoderm induction by fibroblast growth factor. The PCR product was used to clone a 2.3-kilobase pair cDNA representing this transcript, which we have named er1 (early response 1). The er1 cDNA contained a single open reading frame predicted to encode a protein of 493 amino acid residues. A data base homology search revealed that the predicted ER1 amino acid sequence contains three regions of similarity to the rat and human proteins encoded by the metastasis-associated gene, mta1, and two regions of similarity to the Caenorhabditis elegans sequence that is similar to mta1. The fibroblast growth factor-induced increase in er1 steady-state levels was not dependent on de novo protein synthesis, demonstrating that er1 is an immediate-early gene. Northern blot analysis revealed a single 2.8-kilobase pair mRNA that was observed predominantly during the initial cleavage and blastula stages of Xenopus development, with little or no detectable mRNA during subsequent development. Quantitative PCR analysis of early developmental stages showed that er1 peaked during late blastula. Computer-assisted analysis of the predicted ER1 amino acid sequence revealed two putative nuclear localization signals, four highly acidic regions clustered at the N terminus and a proline-rich region located near the C terminus. Subcellular localization by immunocytochemistry revealed that the ER1 protein was targeted exclusively to the nucleus. Transactivation assays using various regions of ER1 fused to the DNA binding domain of GAL4 demonstrated that the N-terminal acidic region is a potent transactivator. These data suggest that ER1 may function as a transcription factor.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
272
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
25591-5
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9325278-3T3 Cells, pubmed-meshheading:9325278-Amino Acid Sequence, pubmed-meshheading:9325278-Animals, pubmed-meshheading:9325278-Base Sequence, pubmed-meshheading:9325278-Caenorhabditis elegans, pubmed-meshheading:9325278-Cloning, Molecular, pubmed-meshheading:9325278-DNA, Complementary, pubmed-meshheading:9325278-Fibroblast Growth Factors, pubmed-meshheading:9325278-Gene Expression, pubmed-meshheading:9325278-Histone Deacetylases, pubmed-meshheading:9325278-Humans, pubmed-meshheading:9325278-Immediate-Early Proteins, pubmed-meshheading:9325278-Mice, pubmed-meshheading:9325278-Molecular Sequence Data, pubmed-meshheading:9325278-Nuclear Proteins, pubmed-meshheading:9325278-Proteins, pubmed-meshheading:9325278-Rats, pubmed-meshheading:9325278-Repressor Proteins, pubmed-meshheading:9325278-Trans-Activators, pubmed-meshheading:9325278-Xenopus Proteins, pubmed-meshheading:9325278-Xenopus laevis
pubmed:year
1997
pubmed:articleTitle
cDNA cloning of a novel, developmentally regulated immediate early gene activated by fibroblast growth factor and encoding a nuclear protein.
pubmed:affiliation
Terry Fox Cancer Research Laboratories, Division of Basic Medical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John's, Newfoundland A1B 3V6, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't