Linked Life Data

9324228

Source:http://linkedlifedata.com/resource/pubmed/id/9324228

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-11-13
pubmed:abstractText
Mesencephalic dopaminergic neurones typically require the presence of serum for their survival in culture. However, the present study, outlines how neurones from the rat ventral mesencephalon (E14-16) were successfully cultured in serum-free, antioxidant-rich Neurobasal medium supplemented with B27 components. Moreover, immunostaining with mouse monoclonal microtubule-associated protein 2 (MAP2) and glial fibrillary acidic protein (GFAP) from day 1 to 7 in vitro revealed these cultures were primarily neuronal (80-95%). Additionally, immunostaining with tyrosine hydroxylase (TH) revealed these cultures contained a relatively constant population of TH-positive neurones (5%) which were presumed to be dopaminergic. These primary cultures offer considerable advantages for the study of mesencephalic, TH-positive, dopaminergic neurones under conditions where milieu can be readily manipulated in the virtual absence of glia and without the confounding influence of serum.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0304-3940
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
233
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
13-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Development and survival of rat embryonic mesencephalic dopaminergic neurones in serum-free, antioxidant-rich primary cultures.
pubmed:affiliation
Department of Pharmacology, Monash University, Clayton, Victoria, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't