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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1997-10-23
|
| pubmed:abstractText |
Using quantitative double-label fluorescence immunocytochemistry and confocal microscopy, we have analysed the pattern of expression of SSTR1-5 in normal rat pituitary somatotrophes. Antipeptide rabbit polyclonal antibodies were produced against the extracellular domains of SSTR1-5. SSTR antigens were colocalized in GH positive cells using rhodamine conjugated secondary antibody for SSTRs and FITC-conjugated secondary antibody for GH. SSTR5 was the predominant subtype which was expressed in 86 +/- 9.7% of GH cells followed by SSTR2 in 42 +/- 6.4% of GH positive cells. SSTR4 and SSTR3 were modestly expressed in 23 +/- 4.7% and 18 +/- 3.2% of somatotrophes respectively whereas SSTR1 was the least expressed subtype occurring in only 5 +/- 1.2% of somatotrophes. These results demonstrate variable expression of the 5 SSTRs in somatotrophes. The preponderance of the SST-28 preferring SSTR5 subtype correlates with the reported higher potency of SST-28 than SST-14 for inhibiting GH secretion.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0013-7227
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
138
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4473-6
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9322965-Animals,
pubmed-meshheading:9322965-Antibodies,
pubmed-meshheading:9322965-Antigens,
pubmed-meshheading:9322965-CHO Cells,
pubmed-meshheading:9322965-Cells, Cultured,
pubmed-meshheading:9322965-Cricetinae,
pubmed-meshheading:9322965-Growth Hormone,
pubmed-meshheading:9322965-Immunohistochemistry,
pubmed-meshheading:9322965-Male,
pubmed-meshheading:9322965-Microscopy, Confocal,
pubmed-meshheading:9322965-Pituitary Gland,
pubmed-meshheading:9322965-Rabbits,
pubmed-meshheading:9322965-Rats,
pubmed-meshheading:9322965-Rats, Inbred Strains,
pubmed-meshheading:9322965-Receptors, Somatostatin,
pubmed-meshheading:9322965-Transfection
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Expression of the five somatostatin receptor (SSTR1-5) subtypes in rat pituitary somatotrophes: quantitative analysis by double-layer immunofluorescence confocal microscopy.
|
| pubmed:affiliation |
Department of Medicine, McGill University, Royal Victoria Hospital and Montreal Neurological Institute, Quebec, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|