Linked Life Data

9322765

Source:http://linkedlifedata.com/resource/pubmed/id/9322765

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1997-10-30
pubmed:databankReference
pubmed:abstractText
The secY gene of Vibrio cholerae has been cloned and the complete nt sequence determined. It codes for a protein of 438 aa residues which functions as a translocator through which proteins cross the inner membrane. It can substitute for the Escherichia coli SecY protein and can suppress the phenotypic traits associated with E. coli secY mutants. The V. cholerae secY gene has about 71 and 83% similarity at the nt and aa levels respectively with the E. coli secY gene. Vibrio cholerae secY, similarly to the E. coli secY, is flanked by the genes encoding the ribosomal large subunit proteins L15 and L36. When expressed from the lac promoter, V. cholerae secY partially complements E. coli secY mutation even in absence of IPTG, while the E. coli secY gene complements only when IPTG is present. Presence of multiple SD sequences and a putative downstream (DS) box imply that the V. cholerae secY gene might have high translational efficiency. A V. cholerae mutant unable to translocate CTB through the inner membrane has been isolated. The secretion deficient phenotype of the mutant can be reversed by introducing the cloned V. cholerae secY gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
196
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
261-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
The secY gene of V. cholerae: identification, cloning and characterization.
pubmed:affiliation
Biophysics Division, Indian Institute of Chemical Biology, Calcutta, India.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't