Linked Life Data

9321466

Source:http://linkedlifedata.com/resource/pubmed/id/9321466

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1997-11-18
pubmed:databankReference
pubmed:abstractText
The Escherichia coli F18 receptor locus (ECF18R) has been genetically mapped to the halothane linkage group on porcine Chromosome (Chr) 6. In an attempt to obtain candidate genes for this locus, we isolated 5 cosmids containing the alpha (1,2)fucosyltransferase genes FUT1, FUT2, and the pseudogene FUT2P from a porcine genomic library. Mapping by fluorescence in situ hybridization placed all these clones in band q11 of porcine Chr 6 (SSC6q11). Sequence analysis of the cosmids resulted in the characterization of an open reading frame (ORF), 1098 bp in length, that is 82.3% identical to the human FUT1 sequence; a second ORF, 1023 bp in length, 85% identical to the human FUT2 sequence; and a third FUT-like sequence thought to be a pseudogene. The FUT1 and FUT2 loci therefore seem to be the porcine equivalents of the human blood group H and Secretor loci. Direct sequencing of the two ORFs in swine being either susceptible or resistant to adhesion and colonization by F18 fimbriated Escherichia coli (ECF18) revealed two polymorphisms at bp 307 (M307) and bp 857 (M857) of the FUT1 ORF. Analysis of these mutations in 34 Swiss Landrace families with 221 progeny showed close linkage with the locus controlling resistance and susceptibility to E. coli F18 adhesion and colonization in the small intestine (ECF18R), and with the locus of the blood group inhibitor S. A high linkage disequilibrium of M307-ECF18R in Large White pigs makes the M307 mutation a good marker for marker-assisted selection of E. coli F18 adhesion-resistant animals in this breed. Whether the FUT1 or possibly the FUT2 gene products are involved in the synthesis of carbohydrate structures responsible for bacterial adhesion remains to be determined.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0938-8990
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
736-41
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9321466-Adhesins, Escherichia coli, pubmed-meshheading:9321466-Amino Acid Sequence, pubmed-meshheading:9321466-Animals, pubmed-meshheading:9321466-Base Sequence, pubmed-meshheading:9321466-Chromosome Mapping, pubmed-meshheading:9321466-Chromosomes, pubmed-meshheading:9321466-Cosmids, pubmed-meshheading:9321466-Fucosyltransferases, pubmed-meshheading:9321466-Genetic Markers, pubmed-meshheading:9321466-Humans, pubmed-meshheading:9321466-In Situ Hybridization, Fluorescence, pubmed-meshheading:9321466-Linkage Disequilibrium, pubmed-meshheading:9321466-Molecular Sequence Data, pubmed-meshheading:9321466-Mutation, pubmed-meshheading:9321466-Open Reading Frames, pubmed-meshheading:9321466-Polymorphism, Genetic, pubmed-meshheading:9321466-Pseudogenes, pubmed-meshheading:9321466-Receptors, Immunologic, pubmed-meshheading:9321466-Sequence Homology, Nucleic Acid, pubmed-meshheading:9321466-Swine
pubmed:year
1997
pubmed:articleTitle
Two alpha(1,2) fucosyltransferase genes on porcine chromosome 6q11 are closely linked to the blood group inhibitor (S) and Escherichia coli F18 receptor (ECF18R) loci.
pubmed:affiliation
Institute of Animal Science, Swiss Federal Institute of Technology, ETH-Zentrum, Zurich, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't