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pubmed:abstractText |
A bacterioferritin (BFR) deletion mutant of Brucella melitensis 16M was generated by gene replacement. The deletion was complemented with a broad-host-range vector carrying the wild-type bfr gene, pBBR-bfr. The survival and growth of the mutant, B. melitensis PAD 2-78, were similar to those of its parental strain in human monocyte-derived macrophages (MDM). These results suggest that BFR is not essential for the intracellular survival of B. melitensis in human MDM.
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pubmed:affiliation |
U. R. Biologie Moléculaire, Laboratoire de Microbiologie et d'Immunologie, Facultés Universitaires Notre Dame de la Paix, Namur, Belgium. Philippe.Denoel@fundp.ac.be
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