| pubmed-article:9316485 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9316485 | lifeskim:mentions | umls-concept:C0021368 | lld:lifeskim |
| pubmed-article:9316485 | lifeskim:mentions | umls-concept:C0085979 | lld:lifeskim |
| pubmed-article:9316485 | lifeskim:mentions | umls-concept:C0458827 | lld:lifeskim |
| pubmed-article:9316485 | lifeskim:mentions | umls-concept:C1456820 | lld:lifeskim |
| pubmed-article:9316485 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:9316485 | pubmed:issue | 3 Pt 1 | lld:pubmed |
| pubmed-article:9316485 | pubmed:dateCreated | 1997-10-23 | lld:pubmed |
| pubmed-article:9316485 | pubmed:abstractText | We have cloned and expressed recombinant guinea pig tumor necrosis factor-alpha (gpTNF-alpha) and examined its inflammatory activities after tracheal instillation in guinea pigs. A 1,071-bp cDNA, including the region encoding the full-length 234-amino acid gpTNF-alpha protein, was cloned from concanavalin A-stimulated guinea pig splenocytes. The 154-amino acid protein corresponding to secreted gpTNF-alpha was expressed as a fusion protein in Escherichia coli, purified by affinity chromatography, and cleaved to yield a 17-kDa protein. gpTNF-alpha had a cytotoxic effect on WEHI 164 cells and was detected by goat anti-murine tumor necrosis factor-alpha (TNF-alpha) antibody in Western blots. Intratracheal instillation of gpTNF-alpha (50-150 ng) caused pronounced and dose-dependent airway eosinophilia. Incubation of gpTNF-alpha with rabbit anti-murine TNF-alpha sera or heating the gpTNF-alpha before instillation reduced bronchoalveolar lavage (BAL) eosinophils to near control levels. Maximum BAL eosinophilia was observed at 24 h, but eosinophil numbers remained significantly above vehicle-treated animals for 72 h. Hence, gpTNF-alpha elicits a pronounced and protracted eosinophil accumulation in the guinea pig lung. | lld:pubmed |
| pubmed-article:9316485 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9316485 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9316485 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9316485 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9316485 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9316485 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9316485 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9316485 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:9316485 | pubmed:issn | 0002-9513 | lld:pubmed |
| pubmed-article:9316485 | pubmed:author | pubmed-author:YoshimuraTT | lld:pubmed |
| pubmed-article:9316485 | pubmed:author | pubmed-author:WestwickJJ | lld:pubmed |
| pubmed-article:9316485 | pubmed:author | pubmed-author:SmithA WAW | lld:pubmed |
| pubmed-article:9316485 | pubmed:author | pubmed-author:WhiteA MAM | lld:pubmed |
| pubmed-article:9316485 | pubmed:author | pubmed-author:WatsonM LML | lld:pubmed |
| pubmed-article:9316485 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9316485 | pubmed:volume | 273 | lld:pubmed |
| pubmed-article:9316485 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9316485 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9316485 | pubmed:pagination | L524-30 | lld:pubmed |
| pubmed-article:9316485 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:9316485 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9316485 | pubmed:articleTitle | Airway inflammation induced by recombinant guinea pig tumor necrosis factor-alpha. | lld:pubmed |
| pubmed-article:9316485 | pubmed:affiliation | School of Pharmacy and Pharmacology, University of Bath, United Kingdom. | lld:pubmed |
| pubmed-article:9316485 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9316485 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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