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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3 Pt 1
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pubmed:dateCreated |
1997-10-23
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pubmed:abstractText |
A cholecystokinin (CCK)-releasing peptide, luminal CCK-releasing factor (LCRF), has been purified from rat jejunal secretion. Amino acid analysis and mass spectral analysis showed that the purified peptide is composed of 70-75 amino acid residues and has a mass of 8,136 Da. Microsequence analysis of LCRF yielded an amino acid sequence for the amino-terminal 41 residues. To determine the biologically active region of the molecule, a peptide was synthesized consisting of the amino-terminal 35 amino acids of LCRF. In this study, intraduodenal infusion of LCRF-(1-35) significantly stimulated pancreatic secretion in conscious rats. The dose-response curves to LCRF-(1-35) and to monitor peptide were similar and biphasic, with higher doses producing submaximal pancreatic secretory responses. The CCK-A receptor antagonist MK-329 abolished the pancreatic secretory response to intraduodenally infused LCRF-(1-35). These results demonstrate that LCRF biological activity is contained within the amino-terminal 35-amino acid portion of LCRF, and this fragment may be useful for investigating the role of LCRF in gastrointestinal function.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0002-9513
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
G754-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9316481-Animals,
pubmed-meshheading:9316481-Benzodiazepinones,
pubmed-meshheading:9316481-Devazepide,
pubmed-meshheading:9316481-Duodenum,
pubmed-meshheading:9316481-Gastrointestinal Hormones,
pubmed-meshheading:9316481-Growth Substances,
pubmed-meshheading:9316481-Hormone Antagonists,
pubmed-meshheading:9316481-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:9316481-Male,
pubmed-meshheading:9316481-Molecular Weight,
pubmed-meshheading:9316481-Pancreas,
pubmed-meshheading:9316481-Pancreatic Juice,
pubmed-meshheading:9316481-Peptide Fragments,
pubmed-meshheading:9316481-Rats,
pubmed-meshheading:9316481-Rats, Wistar,
pubmed-meshheading:9316481-Sincalide
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pubmed:year |
1997
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pubmed:articleTitle |
An amino-terminal fragment of LCRF, LCRF-(1-35), has the same activity as the natural peptide.
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pubmed:affiliation |
Department of Physiology, University of Texas Health Science Center, San Antonio 78284-7756, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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