Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3 Pt 1
pubmed:dateCreated
1997-10-23
pubmed:abstractText
CGP 42112, a high-affinity ligand for angiotensin II AT2 receptors, binds to rat macrophage/microglia lacking AT2 receptors. Here we report that CGP-42112 binds to human monocytes and exerts specific effects. Binding studies revealed a single site, highly specific for CGP-42112, not displaceable by angiotensin II, angiotensin fragments, tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-4, IL-10, transforming growth factor-beta, or lipopolysaccharide (LPS). Incubation of purified human monocytes in serum-free medium with CGP-42112 enhanced, in a dose-dependent manner, cell attachment to fibronectin and collagen-coated dishes as well as matrix metalloproteinase-9 secretion. CGP-42112 did not promote cytokine secretion. In contrast, when added in the presence of low doses of LPS, CGP-42112 reduced the LPS-stimulated secretion of TNF-alpha, IL-1 alpha, IL-1 beta, and IL-6 without affecting IL-10 and decreased the LPS-stimulated matrix metalloproteinase-9 activity. Additionally, CGP-42112 inhibited the increase in protein kinase A activity produced by LPS. Our results indicate that CGP-42112 may modulate monocyte activation through binding to a novel receptor.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/CGP 42112A, http://linkedlifedata.com/resource/pubmed/chemical/Collagenases, http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free, http://linkedlifedata.com/resource/pubmed/chemical/Cytokines, http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6, http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides, http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 9, http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides, http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Angiotensin, Type 2, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Angiotensin, http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
273
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C826-33
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9316402-Animals, pubmed-meshheading:9316402-Binding, Competitive, pubmed-meshheading:9316402-Cell Adhesion, pubmed-meshheading:9316402-Cells, Cultured, pubmed-meshheading:9316402-Collagenases, pubmed-meshheading:9316402-Culture Media, Serum-Free, pubmed-meshheading:9316402-Cytokines, pubmed-meshheading:9316402-Dinoprostone, pubmed-meshheading:9316402-Humans, pubmed-meshheading:9316402-Interleukin-1, pubmed-meshheading:9316402-Interleukin-10, pubmed-meshheading:9316402-Interleukin-6, pubmed-meshheading:9316402-Lipopolysaccharides, pubmed-meshheading:9316402-Matrix Metalloproteinase 9, pubmed-meshheading:9316402-Monocytes, pubmed-meshheading:9316402-Oligopeptides, pubmed-meshheading:9316402-Rats, pubmed-meshheading:9316402-Receptor, Angiotensin, Type 2, pubmed-meshheading:9316402-Receptors, Angiotensin, pubmed-meshheading:9316402-Tumor Necrosis Factor-alpha
pubmed:year
1997
pubmed:articleTitle
CGP-42112 partially activates human monocytes and reduces their stimulation by lipopolysaccharides.
pubmed:affiliation
Section on Pharmacology, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20892, USA.
pubmed:publicationType
Journal Article