Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1997-10-23
pubmed:databankReference
pubmed:abstractText
Polycomb (Pc) is involved in the stable and heritable repression of homeotic gene activity during Drosophila development. Here, we report the identification of a novel human Pc homolog, hPc2. This gene is more closely related to a Xenopus Pc homolog, XPc, than to a previously described human Pc homolog, CBX2 (hPc1). However, the hPc2 and CBX2/hPc1 proteins colocalize in interphase nuclei of human U-2 OS osteosarcoma cells, suggesting that the proteins are part of a common protein complex. To study the functions of the novel human Pc homolog, we generated a mutant protein, delta hPc2, which lacks an evolutionarily conserved C-terminal domain. This C-terminal domain is important for hPc2 function, since the delta hPc2 mutant protein which lacks the C-terminal domain is unable to repress gene activity. Expression of the delta hPc2 protein, but not of the wild-type hPc2 protein, results in cellular transformation of mammalian cell lines as judged by phenotypic changes, altered marker gene expression, and anchorage-independent growth. Specifically in delta hPc2-transformed cells, the expression of the c-myc proto-oncogene is strongly enhanced and serum deprivation results in apoptosis. In contrast, overexpression of the wild-type hPc2 protein results in decreased c-myc expression. Our data suggest that hPc2 is a repressor of proto-oncogene activity and that interference with hPc2 function can lead to derepression of proto-oncogene transcription and subsequently to cellular transformation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-103000, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-1352241, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-1353445, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-1373817, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-1406976, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-1555236, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-1628830, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-1708124, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-1898775, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-1904008, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-1982376, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-2246278, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-2328834, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-2563569, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-2905316, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-3019559, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-3299052, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-3299053, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-3459717, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-3843705, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-3915782, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-7551550, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-7555711, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-7576109, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-7595224, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-7623853, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-7662368, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-7720711, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-7782071, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-7846125, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-7903220, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-7906858, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-7995520, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-8013911, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-8068938, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-8180133, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-8413224, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-8467801, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-8521824, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-8555110, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-8608939, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-8816465, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-9009205, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-9043087, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-9121482, http://linkedlifedata.com/resource/pubmed/commentcorrection/9315667-9199346
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6076-86
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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