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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1997-11-21
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pubmed:abstractText |
The proliferation rate and differentiation state were investigated in porcine inner cell masses (ICMs) and epiblasts in vitro. ICMs isolated from early blastocysts (Day 7 of pregnancy) and epiblasts isolated from preelongated blastocysts (Day 11 of pregnancy) were cultured for up to 5 days in the presence of human leukemia inhibitory factor (hLIF) (1000 U/ml). The proliferation rate was evaluated by determination of the percentage of cells in S-phase. The differentiation state was determined by studying the expression of the stage-specific embryonic antigen-1 (SSEA-1), a marker for undifferentiated murine embryonic stem (ES) cells, and the expression of laminin and cytokeratins 8/18, markers of ES cell differentiation. The staining pattern showed that freshly collected Day 11 epiblasts appeared undifferentiated but rapidly lost this characteristic in vitro. A decrease in the proliferation rate was also observed during culture. This decrease was reduced in the presence of high concentrations of hLIF (optimal concentrations: 5000 U/ml). Conversely, treatment of Day 11 epiblast cells with retinoic acid, an agent known to induce differentiation in murine ES cells, caused a dramatic decrease in the proliferation rate in vitro. In contrast to Day 11 epiblasts, Day 7 ICMs expressed SSEA-1 in vitro and showed a higher proliferation rate (p < 0.01). However, their proliferation rate also decreased during culture and following trypsinization. These results indicate that the undifferentiated characteristics of Day 7 ICMs are more likely to be maintained in vitro than are those of Day 11 epiblasts, which are rapidly committed into early differentiation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD15,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/LIF protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Leukemia Inhibitory Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Lif protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-3363
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
57
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
756-64
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9314577-Animals,
pubmed-meshheading:9314577-Antigens, CD15,
pubmed-meshheading:9314577-Blastocyst,
pubmed-meshheading:9314577-Cell Differentiation,
pubmed-meshheading:9314577-Cell Division,
pubmed-meshheading:9314577-Cells, Cultured,
pubmed-meshheading:9314577-Female,
pubmed-meshheading:9314577-Fibroblasts,
pubmed-meshheading:9314577-Growth Inhibitors,
pubmed-meshheading:9314577-Immunohistochemistry,
pubmed-meshheading:9314577-Interleukin-6,
pubmed-meshheading:9314577-Kinetics,
pubmed-meshheading:9314577-Leukemia Inhibitory Factor,
pubmed-meshheading:9314577-Lymphokines,
pubmed-meshheading:9314577-Mice,
pubmed-meshheading:9314577-Pregnancy,
pubmed-meshheading:9314577-Stem Cells,
pubmed-meshheading:9314577-Swine,
pubmed-meshheading:9314577-Tretinoin
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pubmed:year |
1997
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pubmed:articleTitle |
Proliferation and differentiation of porcine inner cell mass and epiblast in vitro.
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pubmed:affiliation |
INRA, URA-CNRS 1291, Nouzilly, France. florence.wianny@ens-lyon.fr
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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