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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
40
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pubmed:dateCreated |
1997-10-22
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pubmed:abstractText |
The small GTPase Rho is implicated in cytoskeletal rearrangements including stress fiber and focal adhesion formation and in the transcriptional activation of c-fos serum response element. In vitro, Rho-kinase, which is activated by Rho, phosphorylates not only myosin light chain (MLC) (thereby activating myosin ATPase) but also myosin phosphatase, thus inactivating myosin phosphatase. Rho-kinase is involved in the formation of stress fibers and focal adhesions in fibroblasts. Here we show that the expression of constitutively active Rho-kinase increased the level of MLC phosphorylation. The activity of Rho-kinase was necessary for maintaining the vinculin-containing focal adhesions, whereas organized actin stress fibers were not necessary for this. The microinjection of constitutively active Rho-kinase into fibroblasts induced the formation of focal adhesions to some extent under the conditions where organized actin stress fibers were disrupted. The expression of constitutively active Rho-kinase also stimulated the transcriptional activity of c-fos serum response element. These results suggest that Rho-kinase has distinct roles in divergent pathways downstream of Rho, which include MLC phosphorylation leading to stress fiber formation, focal adhesion formation, and gene expression.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochalasin D,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Myosin Light Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Myosins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Response Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Vinculin,
http://linkedlifedata.com/resource/pubmed/chemical/rho-Associated Kinases
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
272
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
25121-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9312122-3T3 Cells,
pubmed-meshheading:9312122-Actins,
pubmed-meshheading:9312122-Animals,
pubmed-meshheading:9312122-Base Sequence,
pubmed-meshheading:9312122-COS Cells,
pubmed-meshheading:9312122-Cell Adhesion,
pubmed-meshheading:9312122-Cell Line,
pubmed-meshheading:9312122-Cytochalasin D,
pubmed-meshheading:9312122-Cytoskeleton,
pubmed-meshheading:9312122-DNA-Binding Proteins,
pubmed-meshheading:9312122-Fibroblasts,
pubmed-meshheading:9312122-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:9312122-Mice,
pubmed-meshheading:9312122-Molecular Sequence Data,
pubmed-meshheading:9312122-Mutagenesis, Insertional,
pubmed-meshheading:9312122-Myosin Light Chains,
pubmed-meshheading:9312122-Myosins,
pubmed-meshheading:9312122-Nuclear Proteins,
pubmed-meshheading:9312122-Oligodeoxyribonucleotides,
pubmed-meshheading:9312122-Phosphorylation,
pubmed-meshheading:9312122-Protein-Serine-Threonine Kinases,
pubmed-meshheading:9312122-Recombinant Fusion Proteins,
pubmed-meshheading:9312122-Serum Response Factor,
pubmed-meshheading:9312122-Spodoptera,
pubmed-meshheading:9312122-Transcription Factors,
pubmed-meshheading:9312122-Transcriptional Activation,
pubmed-meshheading:9312122-Transfection,
pubmed-meshheading:9312122-Vinculin,
pubmed-meshheading:9312122-rho-Associated Kinases
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pubmed:year |
1997
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pubmed:articleTitle |
Cytoskeletal rearrangements and transcriptional activation of c-fos serum response element by Rho-kinase.
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pubmed:affiliation |
Division of Signal Transduction, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-01, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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