rdf:type |
|
lifeskim:mentions |
umls-concept:C0019704,
umls-concept:C0035679,
umls-concept:C0086418,
umls-concept:C0205225,
umls-concept:C0205263,
umls-concept:C0441655,
umls-concept:C0663446,
umls-concept:C1413181,
umls-concept:C1514562,
umls-concept:C1707271,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
10
|
pubmed:dateCreated |
1997-10-20
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pubmed:abstractText |
Tat protein mediates transactivation of human immunodeficiency virus type 1 (HIV-1), which results in more-efficient transcript elongation. Since phosphorylation of C-terminal domain (CTD) of RNA polymerase II correlates with its enhanced processivity, we studied the properties of a Tat-associated CTD kinase derived from mitogenically stimulated human primary T lymphocytes (TTK). TTK binds to full-length Tat and specifically phosphorylates CTD and CDK2. This dual kinase activity is characteristic of CDK-activating kinase (CAK). The CTD kinase activity is induced upon mitogenic stimulation of primary T lymphocytes. Fractionation of T-cell lysate demonstrates that Tat-associated CTD kinase activity elutes in two peaks. About 60% of Tat-associated CTD kinase copurifies with CDK2 kinase activity and contains the CAK components CDK7 and cyclin H. The rest of Tat-associated kinase is free of CDK2 kinase activity and the CAK components and thus may represent a novel CTD kinase. The kinase activities of TTK are blocked by the adenosine analog 5,6-dichloro-1-beta-D-ribofuranosyl-benzimidazole (DRB) as well as by the kinase inhibitor H8 at concentrations known to block transcript elongation. Importantly, the Tat-associated kinase markedly induced CAK. We suggest that the mechanism of Tat-mediated processive transcription of the HIV-1 promoter includes a Tat-associated CAK activator.
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pubmed:grant |
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-1450662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-1547941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-1559613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-1756726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-1925601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-1956324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-2023953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-2331748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-2376591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-2422271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-2553266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-2708335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-2849509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-2914905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-2999785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-3019009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-7533895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-7553872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-7572393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-7574492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-7592583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-7609087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-7638159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-7711060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-7835343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-7853496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-7885450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-7979253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-8001135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-8001136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-8015613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-8069918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-8121496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-8171003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-8191958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-8487311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-8521818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-8600022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-8602364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-8637904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-8700889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-8764062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-8849451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-8934526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9311822-9184228
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CDC2-CDC28 Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/CDK2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/Positive Transcriptional...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Polymerase II,
http://linkedlifedata.com/resource/pubmed/chemical/carboxy-terminal domain kinase,
http://linkedlifedata.com/resource/pubmed/chemical/cyclin-dependent kinase-activating...,
http://linkedlifedata.com/resource/pubmed/chemical/tat Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
|
pubmed:issn |
0022-538X
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
71
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7436-41
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
|