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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1997-10-23
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pubmed:abstractText |
Different ovarian follicle counting procedures were investigated to reduce labor while retaining statistical power. Intact ovaries of untreated CD-1 mice (20/group) from National Toxicology Program Reproductive Assessment by Continuous Breeding (RACB) studies were serially sectioned at 6 microm. Mean numbers of small and growing follicles were used to assess sampling efficiency. In 10 mice per group, comparisons were made between 10% nonrandom samples from every 10th section starting at either the first or sixth section having follicles (approximately 40 sections per ovary). These 10% counts were compared with 5% (20 sections) and 20% (80 sections) nonrandom samples and with 1% (4 sections), 5%, or 10% random samples from the same 10 animals. For two studies, a 10% nonrandom sample was analyzed from 20 mice per group. Follicle counts for each group were comparable regardless of the sampling paradigm. Four to 10 animals provided 90% confidence that a 20% difference in mean counts would be detected. The 1% sample had a larger error term and, thus, slightly reduced statistical power. These data suggest that follicle counts from 1% or 5% random samples may provide a suitable screen for ovarian toxicity.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:issn |
0890-6238
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
689-96
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9311577-Animals,
pubmed-meshheading:9311577-Cell Count,
pubmed-meshheading:9311577-Data Interpretation, Statistical,
pubmed-meshheading:9311577-Female,
pubmed-meshheading:9311577-Histological Techniques,
pubmed-meshheading:9311577-Male,
pubmed-meshheading:9311577-Mice,
pubmed-meshheading:9311577-Mice, Inbred ICR,
pubmed-meshheading:9311577-Ovarian Follicle,
pubmed-meshheading:9311577-Random Allocation,
pubmed-meshheading:9311577-Reproducibility of Results,
pubmed-meshheading:9311577-Research Design
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pubmed:articleTitle |
Influence of sampling on the reproducibility of ovarian follicle counts in mouse toxicity studies.
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pubmed:affiliation |
Pathology Associates International (an SAIC Company), National Center for Toxicological Research, Jefferson, Arkansas 72079, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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