Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-10-23
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D16226, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D32069, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D50453, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D78137, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D90913, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L01134, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U06451, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U08813, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U60282, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U66088, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U72716, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X06653, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X86084, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z73898, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z81049, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P10502, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P11170, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P13866, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P26429, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P26430, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P31448, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P31636, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P31637, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P31639, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P33413, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P53790, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P53791, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P53792, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P53793, http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P53794
pubmed:abstractText
Homologues of the Na+/glucose cotransporter, the SGLT family, include sequences of mammalian, eubacterial, yeast, insect and nematode origin. The cotransported substrates are sugars, inositol, proline, pantothenate, iodide, urea and undetermined solutes. It is reasonable to expect that the SGLT family members share a similar or identical topology of membrane spanning elements, by virtue of their common ancestry and similar coupling of solute transport to downhill sodium flux. Here we examine their membrane topologies as deduced from diverse analyses of their primary sequences, and from their sequence correlations with the experimentally determined topology of the human Na+/ glucose contransporter SGLT1. Our analyses indicate that all family members share a common core of 13 transmembrane helices, but that some, like SGLT1 itself, have one additional span appended to the C-terminus, and still others, two. One bacterial member incorporates an additional span at the N-terminus. Sequence comparisons indicative of common ancestry of the SGLT and the [Na+ + Cl-] transporter families are introduced, and evaluated in light of their topologies. New evidence concerning the previously asserted common ancestry of SGLT1 and an N-acetylglucosamine permease of the bacterial phosphotransferase system is considered. Finally, we analyze observations which lead us to conjecture that the experimental strategy most commonly employed to reveal the topology of bacterial transporters (i.e., the fusion of reporter enzymes such as phoA alkaline phosphatase, beta-lactamase or beta-galactosidase, to progressively C-truncated fragments of the transporter) has often instead so perturbed local topology as to have entirely missed pairs of adjacent membrane spans.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-2631
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
159
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-20
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Membrane topology motifs in the SGLT cotransporter family.
pubmed:affiliation
Department of Physiology, UCLA School of Medicine 90095-1751, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Review