Linked Life Data

9307299

Source:http://linkedlifedata.com/resource/pubmed/id/9307299

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
1997-10-20
pubmed:abstractText
We examined the expression of glial- and neuronal-specific mRNAs within human gliomas using in situ hybridization. We found that low-grade astrocytomas contained a high number of proteolipid protein (PLP) mRNA-positive cells and that the number of PLP-stained cells decreased markedly with increasing tumor grade. Interestingly, the ratio of PLP mRNA-stained cells:myelin basic protein (MBP) mRNA-stained cells in normal white matter and low-grade astrocytoma was about 2:1 but approached 1:1 with increasing tumor grade. This parameter appeared to be a good indicator of tumor infiltration in astrocytomas, so we tested this in the analysis of other gliomas. Unlike astrocytomas, oligodendrogliomas were found consistently to contain few PLP mRNA- or MBP mRNA-expressing cells. In contrast, gemistocytic astrocytomas, typically highly invasive tumors, contained high numbers of PLP-positive cells and a ratio of PLP mRNA:MBP mRNA-stained cells of about 1.5:1, similar to low-grade astrocytomas. Nonradioactive in situ hybridization also enabled the morphological identification of specific cells. For example, gemistocytic astrocytes, which were found to be strongly vimentin mRNA positive, contained little glial fibrillary acidic protein mRNA and did not stain for PLP or MBP mRNAs. Neuronal mRNAs, such as neurofilament 68, were observed in small numbers of entrapped neurons within gliomas but were uninformative with respect to predicting tumor grade. Our results suggest that oligodendrocytes survive low-grade tumor infiltration and that glial tumor cells, unlike cell lines derived from them, do not express oligodendrocyte or neuronal mRNAs. In addition, the expression of mRNAs for the two major myelin protein genes, PLP and MBP, could be used to predict the grade and extent of tumor infiltration in astrocytomas.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4098-104
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:9307299-Astrocytoma, pubmed-meshheading:9307299-Brain Neoplasms, pubmed-meshheading:9307299-Gene Expression Regulation, Neoplastic, pubmed-meshheading:9307299-Glial Fibrillary Acidic Protein, pubmed-meshheading:9307299-Glioma, pubmed-meshheading:9307299-Humans, pubmed-meshheading:9307299-In Situ Hybridization, pubmed-meshheading:9307299-Keratins, pubmed-meshheading:9307299-Myelin Basic Proteins, pubmed-meshheading:9307299-Myelin Proteolipid Protein, pubmed-meshheading:9307299-Neurofilament Proteins, pubmed-meshheading:9307299-Neuroglia, pubmed-meshheading:9307299-Neurons, pubmed-meshheading:9307299-Oligodendroglia, pubmed-meshheading:9307299-Oligodendroglioma, pubmed-meshheading:9307299-RNA, Messenger, pubmed-meshheading:9307299-RNA, Neoplasm, pubmed-meshheading:9307299-Vimentin
pubmed:year
1997
pubmed:articleTitle
Expression of oligodendrocytic mRNAs in glial tumors: changes associated with tumor grade and extent of neoplastic infiltration.
pubmed:affiliation
Mental Retardation Research Center, University of California, Los Angeles, School of Medicine, 90024, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't