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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1997-10-29
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pubmed:databankReference |
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pubmed:abstractText |
BP-80 is a type I integral membrane protein abundant in pea (Pisum sativum) clathrin-coated vesicles (CCVs) that binds with high affinity to vacuole-targeting determinants containing asparagine-proline-isoleucine-arginine. Here we present results from cDNA cloning and studies of its intracellular localization. Its sequence and sequences of homologs from Arabidopsis, rice (Oryza sativa), and maize (Zea mays) define a novel family of proteins unique to plants that is highly conserved in both monocotyledons and dicotyledons. The BP-80 protein is present in dilated ends of Golgi cisternae and in "prevacuoles," which are small vacuoles separate from but capable of fusing with lytic vacuoles. Its cytoplasmic tail contains a Tyr-X-X-hydrophobic residue motif associated with transmembrane proteins incorporated into CCVs. When transiently expressed in tobacco (Nicotiana tabacum) suspension-culture protoplasts, a truncated form lacking transmembrane and cytoplasmic domains was secreted. These results, coupled with previous studies of ligand-binding specificity and pH dependence, strongly support our hypothesis that BP-80 is a vacuolar sorting receptor that trafficks in CCVs between Golgi and a newly described prevacuolar compartment.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-1392605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-1438303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-1498598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-16666870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-16667086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-1739969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-1821765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-1841724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-1992474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-2199455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-2545438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-2826439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-3266596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-3327686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-7559754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-7569928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-7593183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-7606779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-7750515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-8130797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-8159760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-8187177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-8278490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-8509395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-8509446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-8518558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-8548289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-8787025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-8918456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-8923215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9306690-9225474
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0032-0889
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
115
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
29-39
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:9306690-Amino Acid Sequence,
pubmed-meshheading:9306690-Clathrin,
pubmed-meshheading:9306690-Cloning, Molecular,
pubmed-meshheading:9306690-Genes, Plant,
pubmed-meshheading:9306690-Golgi Apparatus,
pubmed-meshheading:9306690-Immunohistochemistry,
pubmed-meshheading:9306690-Microscopy, Immunoelectron,
pubmed-meshheading:9306690-Molecular Sequence Data,
pubmed-meshheading:9306690-Peas,
pubmed-meshheading:9306690-Plant Proteins,
pubmed-meshheading:9306690-Plants, Genetically Modified,
pubmed-meshheading:9306690-Receptors, Cell Surface,
pubmed-meshheading:9306690-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:9306690-Sequence Homology, Amino Acid,
pubmed-meshheading:9306690-Vacuoles,
pubmed-meshheading:9306690-Vesicular Transport Proteins
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pubmed:year |
1997
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pubmed:articleTitle |
Molecular cloning and further characterization of a probable plant vacuolar sorting receptor.
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pubmed:affiliation |
Biochemistry Department, University of Missouri, Columbia 65211, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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