|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
16
|
|
pubmed:dateCreated |
1997-10-17
|
|
pubmed:abstractText |
SacY belongs to a family of, at present, seven bacterial transcriptional antiterminators. The RNA-binding and antitermination capacity of SacY resides in the 55 amino acids at the N-terminal [SacY(1-55)]. The crystal structure at 2 A resolution shows that SacY(1-55) forms a dimer in the crystal, in accordance with the NMR solution structure. The structure of the monomer is a four-stranded beta-sheet with a simple beta1beta2beta3beta4 topology. One side of the sheet is covered by a long surface loop and the other side forms the dimer interface. The dimer is stabilized by the orthogonal stacking of the two beta-sheets. The crystal structure is in excellent agreement with the NMR solution structure (r.m.s. distance for C alpha coordinates is 1.3 A). The structure of SacY(1-55) reveals a new RNA-binding motif.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305644-1279678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305644-15299555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305644-1920411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305644-2147232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305644-2428811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305644-480354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305644-7574494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305644-7628456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305644-7628457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305644-7746868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305644-7937067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305644-7984237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305644-8602269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305644-8612276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305644-8696973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305644-9305643
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0261-4189
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
16
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
5030-6
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:9305644-Amino Acid Sequence,
pubmed-meshheading:9305644-Bacillus subtilis,
pubmed-meshheading:9305644-Bacterial Proteins,
pubmed-meshheading:9305644-Base Sequence,
pubmed-meshheading:9305644-Crystallography, X-Ray,
pubmed-meshheading:9305644-Dimerization,
pubmed-meshheading:9305644-Escherichia coli,
pubmed-meshheading:9305644-Gene Expression,
pubmed-meshheading:9305644-Models, Molecular,
pubmed-meshheading:9305644-Molecular Sequence Data,
pubmed-meshheading:9305644-Nucleic Acid Conformation,
pubmed-meshheading:9305644-Protein Conformation,
pubmed-meshheading:9305644-Protein Structure, Secondary,
pubmed-meshheading:9305644-RNA, Bacterial,
pubmed-meshheading:9305644-RNA-Binding Proteins,
pubmed-meshheading:9305644-Recombinant Proteins,
pubmed-meshheading:9305644-Transcription Factors
|
|
pubmed:year |
1997
|
|
pubmed:articleTitle |
Crystal structure of a new RNA-binding domain from the antiterminator protein SacY of Bacillus subtilis.
|
|
pubmed:affiliation |
Centre de Biochimie Structurale, CNRS-U9955, INSERM-U414, Montpellier, France. herman@tome.cbs.univ-montp1.fr
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
