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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1998-4-21
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pubmed:abstractText |
The contribution of several vasoactive mediators such as histamine, serotonin, bradykinin, arachidonic acid metabolites and PAF to vascular permeability changes was determined in a rat model of acute endotoxemia. Lipopolysaccharide (10-40 mg/kg, i.v.) from E. coli 0127:B8 (LPS) elicited an increase in Evans blue extravasation in trachea, thymus, seminal vesicle and stomach, whereas other organs remained unaffected. LPS (25 mg/kg)-induced extravasation was not inhibited by intravenous pretreatment with histamine (H1) antagonist mepyramine (5 mg/kg) or bradykinin (B2) antagonist HOE-140 (0.1 mg/kg), whereas other standard drugs selectively inhibited leakage in particular tissues, e.g. the cyclooxygenase inhibitor indomethacin (5 mg/kg) in trachea (78%) and seminal vesicle (64%), the serotonin and H1 antagonists cyproheptadine (2 mg/kg) in trachea (88%) and stomach (56%) and the dual cyclooxygenase/lipoxygenase inhibitor phenidone (10 mg/kg) in seminal vesicle (87%). PAF antagonists lexipafant and UR-12460 (10 mg/kg), but not apafant, potently inhibited extravasation in trachea (59, 84%) and seminal vesicle (81, 78%) and in stomach only UR-12460 (52%), whereas all of them were ineffective in thymus. When extravasation was induced by PAF (4 micrograms/kg) a low dose (0.1 mg/kg) of the three PAF antagonists strongly reduced extravasation in thymus and seminal vesicle, whereas lexipafant and UR-12460 did so in trachea (82, 100%) and only lexipafant in stomach (100%). Mepyramine, cyproheptadine, HOE-140 and indomethacin did not inhibit the effect of PAF, whereas phenidone inhibited it by 58% in trachea. These results suggest that most of the LPS-induced increase in vascular permeability is mediated by secondary vasoactive mediators among which PAF plays a pivotal role, although their relative contribution may vary from tissue to tissue.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents...,
http://linkedlifedata.com/resource/pubmed/chemical/Blood Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Indomethacin,
http://linkedlifedata.com/resource/pubmed/chemical/Leucine,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Piperazines,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Activating Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrazoles,
http://linkedlifedata.com/resource/pubmed/chemical/UR 12460,
http://linkedlifedata.com/resource/pubmed/chemical/lexipafant,
http://linkedlifedata.com/resource/pubmed/chemical/phenidone
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0929-7855
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
31-45
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:9302653-Animals,
pubmed-meshheading:9302653-Anti-Inflammatory Agents, Non-Steroidal,
pubmed-meshheading:9302653-Blood Proteins,
pubmed-meshheading:9302653-Capillary Permeability,
pubmed-meshheading:9302653-Dose-Response Relationship, Drug,
pubmed-meshheading:9302653-Exudates and Transudates,
pubmed-meshheading:9302653-Imidazoles,
pubmed-meshheading:9302653-Indomethacin,
pubmed-meshheading:9302653-Leucine,
pubmed-meshheading:9302653-Lipopolysaccharides,
pubmed-meshheading:9302653-Lipoxygenase Inhibitors,
pubmed-meshheading:9302653-Male,
pubmed-meshheading:9302653-Piperazines,
pubmed-meshheading:9302653-Platelet Activating Factor,
pubmed-meshheading:9302653-Pyrazoles,
pubmed-meshheading:9302653-Rats,
pubmed-meshheading:9302653-Rats, Sprague-Dawley
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pubmed:year |
1997
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pubmed:articleTitle |
Effect of endotoxin and platelet-activating factor on rat vascular permeability: role of vasoactive mediators.
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pubmed:affiliation |
Department of Pharmacology, J. Uriach & Cia Laboratories, Barcelona, Spain.
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pubmed:publicationType |
Journal Article
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