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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1997-10-9
|
| pubmed:abstractText |
Medicago sativa (lucerne) is used as a traditional plant treatment of diabetes. In the present study, administration of lucerne in the diet (62.5 g/kg) and drinking water (2.5 g/l) reduced the hyperglycaemia of streptozotocin-diabetic mice. An aqueous extract of lucerne (1 mg/ml) stimulated 2-deoxy-glucose transport (1.8-fold), glucose oxidation (1.7-fold) and incorporation of glucose into glycogen (1.6-fold) in mouse abdominal muscle. In acute 20 min tests, 0.25-1 mg/ml aqueous extract of lucerne evoked a stepwise 2.5-6.3-fold stimulation of insulin secretion from the BRIN-BD11 pancreatic B-cell line. This effect was abolished by 0.5 mM-diazoxide, and prior exposure to extract did not affect subsequent stimulation of insulin secretion by 10 mM-L-alanine, thereby negating a detrimental effect on cell viability. The effect of extract was potentiated by 16.7 mM-glucose and by 1 mM-3-isobutyl-1-methylxanthine. L-Alanine (10 mM) and a depolarizing concentration of KCl (25 mM) did not augment the insulin-releasing activity of lucerne. Activity of the extract was found to be heat stable and largely acetone insoluble, and was enhanced by exposure to acid and alkali (0.1 M-HCl and NaOH) but decreased 25% with dialysis to remove components with molecular mass < 2000 Da. Sequential extraction with solvents revealed insulin-releasing activity in both methanol and water fractions indicating a cumulative effect of more than one extract constituent. The results demonstrate the presence of antihyperglycaemic, insulin-releasing and insulin-like activity in the traditional antidiabetic plant, Medicago sativa.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-Methyl-3-isobutylxanthine,
http://linkedlifedata.com/resource/pubmed/chemical/Antihypertensive Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyglucose,
http://linkedlifedata.com/resource/pubmed/chemical/Diazoxide,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Glycogen,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphodiesterase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Extracts
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0007-1145
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
78
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
325-34
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9301421-1-Methyl-3-isobutylxanthine,
pubmed-meshheading:9301421-Animals,
pubmed-meshheading:9301421-Antihypertensive Agents,
pubmed-meshheading:9301421-Biological Transport,
pubmed-meshheading:9301421-Culture Techniques,
pubmed-meshheading:9301421-Deoxyglucose,
pubmed-meshheading:9301421-Diabetes Mellitus, Experimental,
pubmed-meshheading:9301421-Diazoxide,
pubmed-meshheading:9301421-Glucose,
pubmed-meshheading:9301421-Glycogen,
pubmed-meshheading:9301421-Insulin,
pubmed-meshheading:9301421-Islets of Langerhans,
pubmed-meshheading:9301421-Male,
pubmed-meshheading:9301421-Medicago sativa,
pubmed-meshheading:9301421-Medicine, Traditional,
pubmed-meshheading:9301421-Mice,
pubmed-meshheading:9301421-Muscle, Skeletal,
pubmed-meshheading:9301421-Phosphodiesterase Inhibitors,
pubmed-meshheading:9301421-Phytotherapy,
pubmed-meshheading:9301421-Plant Extracts,
pubmed-meshheading:9301421-Stimulation, Chemical
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Pancreatic and extra-pancreatic effects of the traditional anti-diabetic plant, Medicago sativa (lucerne).
|
| pubmed:affiliation |
School of Biomedical Sciences, University of Ulster, Coleraine.
|
| pubmed:publicationType |
Journal Article
|