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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8
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pubmed:dateCreated |
1998-1-6
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pubmed:abstractText |
A sensitive and rapid high-performance liquid chromatographic (HPLC) method was developed and used for the simultaneous determination of bilirubin and biliverdin in pericardial fluid samples collected from broilers at a poultry inspection site. A photodiode array detector distinguishing the bilirubin (UV 450 nm) and biliverdin (365 nm) was used as an analytical detector for HPLC system. An internal-surface reversed-phase silica support column was used, and the mobile phase consisted of acetonitrile: 0.5 M Tris HCl buffer (20:80, pH 7.2). Bilirubin was detected from all of the jaundiced pericardial fluid samples, and a small amount of biliverdin was detected with bilirubin in some samples. These jaundiced broilers had hepatic or bile duct lesions similar to those found in edible animals. From these results, a working definition of jaundiced broilers for poultry inspection sites was suggested: bilirubin is detectable from pericardial fluid and the carcass is in a state of yellow color change.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0916-7250
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
677-80
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:9300364-Animals,
pubmed-meshheading:9300364-Bilirubin,
pubmed-meshheading:9300364-Biliverdine,
pubmed-meshheading:9300364-Chickens,
pubmed-meshheading:9300364-Cholestasis,
pubmed-meshheading:9300364-Chromatography, High Pressure Liquid,
pubmed-meshheading:9300364-Disease Outbreaks,
pubmed-meshheading:9300364-Hepatitis, Animal,
pubmed-meshheading:9300364-Humans,
pubmed-meshheading:9300364-Japan,
pubmed-meshheading:9300364-Jaundice,
pubmed-meshheading:9300364-Liver,
pubmed-meshheading:9300364-Pericardium,
pubmed-meshheading:9300364-Poultry Diseases
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pubmed:year |
1997
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pubmed:articleTitle |
High-performance liquid chromatographic analysis of bilirubin and biliverdin from jaundiced broilers.
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pubmed:affiliation |
Department of Veterinary Public Health, Faculty of Agriculture, Miyazaki University, Japan.
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pubmed:publicationType |
Journal Article
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