Linked Life Data

9300241

Source:http://linkedlifedata.com/resource/pubmed/id/9300241

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1997-11-14
pubmed:abstractText
A CD8 T-cell clone (YNK1.3) generated from acutely diabetic NOD mouse islets, showed proliferation and cytotoxicity when challenged with NOD and BALB/c islet cells and NOD-derived insulinoma cells. When 1-2 x 10(7) YNK1.3 cells were administered to 7-10-day-old NOD mice, the cells transferred overt diabetes very rapidly in each of the 16 recipients within 4 days of cell transfer. However, of 14 recipients receiving YNK 1.3 cells above 14 days of age none became diabetic. Fluorescent dye-labelled YNK1.3 cells extensively accumulated in the islets by 36 h after transfer in 7-day-old NOD recipients, while no significant insulitis was seen in 21-day-old recipients. Over half of NOD-scid recipients (5/9) rapidly became diabetic within 5 days after transfer of 1-2 x 10(7) YNK1.3 cells at 7 days of age, whereas only one of 12 recipients over 14 days of age became diabetic. Furthermore, YNK1.3 cells also transferred diabetes to H-2Kd-matched very young BALB/c-scid and CB17-scid mice, but not to C57BL/6-scid mice. Thus, optimally activated islet-specific CD8 T-cell clones are able to rapidly transfer diabetes to NOD and MHC class I compatible scid mice when a large enough number is administered at 7 days of age. Administration of monoclonal antibodies against adhesion molecules involved in the trafficking of lymphocytes from the circulation into the inflammatory tissues, could not prevent the cellular infiltration of YNK1.3 cells into the islets in 7-day-old NOD recipients. The results indicate that islet cells in the mouse around 7 days of age are generally susceptible to cytotoxic CD8 T cells, suggesting, therefore, that CD8 T cells may play an important role in the initiation of autoimmune diabetes in NOD mice.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0012-186X
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1044-52
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9300241-Adoptive Transfer, pubmed-meshheading:9300241-Animals, pubmed-meshheading:9300241-Antibodies, Monoclonal, pubmed-meshheading:9300241-Autoimmune Diseases, pubmed-meshheading:9300241-CD8-Positive T-Lymphocytes, pubmed-meshheading:9300241-Cell Adhesion Molecules, pubmed-meshheading:9300241-Cell Line, pubmed-meshheading:9300241-Clone Cells, pubmed-meshheading:9300241-Diabetes Mellitus, Experimental, pubmed-meshheading:9300241-Histocompatibility Antigens Class I, pubmed-meshheading:9300241-Islets of Langerhans, pubmed-meshheading:9300241-Mice, pubmed-meshheading:9300241-Mice, Inbred BALB C, pubmed-meshheading:9300241-Mice, Inbred C57BL, pubmed-meshheading:9300241-Mice, Inbred NOD, pubmed-meshheading:9300241-Mice, SCID, pubmed-meshheading:9300241-T-Lymphocytes, Cytotoxic
pubmed:year
1997
pubmed:articleTitle
CD8 cytotoxic T-cell clone rapidly transfers autoimmune diabetes in very young NOD and MHC class I-compatible scid mice.
pubmed:affiliation
Second Department of Internal Medicine, Kobe University School of Medicine, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't