Linked Life Data

9299571

Source:http://linkedlifedata.com/resource/pubmed/id/9299571

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-10-16
pubmed:abstractText
Both the protein and mRNA levels of cathepsin L in SR-3Y1-2, v-src-transformed 3Y1 cell lines were higher than in 3Y1 cells. Results of CAT assays suggested that the v-src responsible region in the cathepsin L gene localizes to 300 bp in the 5'-upstream region and 60 bp in the first exon. DNaseI footprinting analyses showed that transcription factors bind to the region from 29bp to 55bp from the transcription start site. This region contains a CAAT-box and a 5'-GGCGGGGGCGG-3' sequence containing two repeat copies of consensus Sp-1 binding sites, overlapping a consensus Egr family binding site. DNA band shift assays showed that Sp-1 and NF-1 binding proteins bind to this region in 3Y1 cells while Egr family protein binds in SR-3Y1-2 cells. These results suggest that Egr family proteins are involved in the activation of the cathepsin L gene in SR-3Y1-2 cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 1997 Academic Press.
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
238
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
665-9
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Egr family member proteins are involved in the activation of the cathepsin L gene in v-src-transformed cells.
pubmed:affiliation
Department of Biochemistry, Juntendo University School of Medicine, Tokyo, 2-1-1 Hongo, Bunkyo-ku, 113, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't