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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1997-10-15
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pubmed:abstractText |
The eubacterium Thermus thermophilus expresses terminal oxidases of the ba3- and caa3-type. The soluble cytochrome-c552 of this organism has been isolated by a new method and characterized. In contrast to previous studies, but in line with coexpression at low aeration, the cytochrome was unambiguously identified as the substrate of the ba3-oxidase. In the presence of TMPD and ascorbate, biphasic Eadie-Hofstee plots with kmax = 250 s-1 at 25 degrees C are observed upon addition of cytochrome-c552. Surprisingly, the caa3-oxidase with its single covalently bound cytochrome-c also exhibits a biphasic redox activity with kmax = 185 s-1 in the presence of TMPD and ascorbate only. Further addition of cytochrome-c552 does not lead to enhanced activity. Crystals of cytochrome-c552 were obtained by vapor diffusion using the sitting-drop method in the presence of ammonium sulfate as precipitant. They diffract to 1.28 A resolution using synchrotron radiation. The structure has been solved by MAD phasing.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
237
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
572-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9299406-Amino Acid Sequence,
pubmed-meshheading:9299406-Ascorbic Acid,
pubmed-meshheading:9299406-Chromatography, Gel,
pubmed-meshheading:9299406-Chromatography, Ion Exchange,
pubmed-meshheading:9299406-Crystallization,
pubmed-meshheading:9299406-Crystallography, X-Ray,
pubmed-meshheading:9299406-Cytochrome b Group,
pubmed-meshheading:9299406-Cytochrome c Group,
pubmed-meshheading:9299406-Electron Transport Complex IV,
pubmed-meshheading:9299406-Fermentation,
pubmed-meshheading:9299406-Substrate Specificity,
pubmed-meshheading:9299406-Thermus thermophilus,
pubmed-meshheading:9299406-Ultrafiltration
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pubmed:year |
1997
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pubmed:articleTitle |
Cytochrome-c552 from Thermus thermophilus: a functional and crystallographic investigation.
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pubmed:affiliation |
Institut für Biochemie, Klinikum der RWTH Aachen, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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