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pubmed-article:9298585pubmed:abstractTextThe 31P NMR spectra of germinating maize seeds showed a single broad resonance that shifted its position as germination proceeded (studied between 0 and 10 days). The resonance was shown to originate from the phosphate groups of phytine (Mg2+, Ca2+ and K+ salt of myoinositol hexakisphosphate) in a subcellular compartment of the embryo scutellar cells. A series of calibration curves for the chemical shift dependence of the phytate resonance in the presence of Mg2+ and Ca2+ were constructed. These calibration curves allowed us to determine that an acidification of the phytate containing compartment in the seed embryo takes place, reaching a minimum at about pH 4 after three days of germination. This acidification could be important in allowing phytate solubilization for export to growing parts of the maize seedling.lld:pubmed
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pubmed-article:9298585pubmed:authorpubmed-author:AlonsoJJlld:pubmed
pubmed-article:9298585pubmed:authorpubmed-author:GasparovicCClld:pubmed
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pubmed-article:9298585pubmed:pagination609-20lld:pubmed
pubmed-article:9298585pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:9298585pubmed:year1997lld:pubmed
pubmed-article:9298585pubmed:articleTitleMeasurement of intracellular pH of maize seeds (Zea mays) during germination by 31P nuclear magnetic resonance spectroscopy.lld:pubmed
pubmed-article:9298585pubmed:affiliationDepartament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain.lld:pubmed
pubmed-article:9298585pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:9298585pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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