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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1997-10-20
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pubmed:abstractText |
The 31P NMR spectra of germinating maize seeds showed a single broad resonance that shifted its position as germination proceeded (studied between 0 and 10 days). The resonance was shown to originate from the phosphate groups of phytine (Mg2+, Ca2+ and K+ salt of myoinositol hexakisphosphate) in a subcellular compartment of the embryo scutellar cells. A series of calibration curves for the chemical shift dependence of the phytate resonance in the presence of Mg2+ and Ca2+ were constructed. These calibration curves allowed us to determine that an acidification of the phytate containing compartment in the seed embryo takes place, reaching a minimum at about pH 4 after three days of germination. This acidification could be important in allowing phytate solubilization for export to growing parts of the maize seedling.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jul
|
pubmed:issn |
0145-5680
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
43
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
609-20
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9298585-Cell Compartmentation,
pubmed-meshheading:9298585-Germination,
pubmed-meshheading:9298585-Hydrogen-Ion Concentration,
pubmed-meshheading:9298585-Magnetic Resonance Spectroscopy,
pubmed-meshheading:9298585-Phosphorus Isotopes,
pubmed-meshheading:9298585-Phytic Acid,
pubmed-meshheading:9298585-Seeds,
pubmed-meshheading:9298585-Zea mays
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pubmed:year |
1997
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pubmed:articleTitle |
Measurement of intracellular pH of maize seeds (Zea mays) during germination by 31P nuclear magnetic resonance spectroscopy.
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pubmed:affiliation |
Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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