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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1997-10-29
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pubmed:abstractText |
The selection of false-positive clones in differential display PCR (DDRT-PCR) represents a formidable drawback to this otherwise powerful technique of detecting subtle differences between cell types. DDRT-PCR performed with cDNAs generated by two different reverse transcriptases from the same RNA doubles the total number of reactions; nevertheless, false-positive clones arising from small differences between RNA preparations are easily distinguished from differentially expressed transcripts.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0736-6205
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
462-4, 466, 468
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9298217-Amyloid beta-Protein Precursor,
pubmed-meshheading:9298217-Animals,
pubmed-meshheading:9298217-COS Cells,
pubmed-meshheading:9298217-DNA, Complementary,
pubmed-meshheading:9298217-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9298217-False Positive Reactions,
pubmed-meshheading:9298217-Genetic Vectors,
pubmed-meshheading:9298217-Metallothionein,
pubmed-meshheading:9298217-Moloney murine leukemia virus,
pubmed-meshheading:9298217-Polymerase Chain Reaction,
pubmed-meshheading:9298217-RNA, Catalytic,
pubmed-meshheading:9298217-RNA, Messenger,
pubmed-meshheading:9298217-RNA-Directed DNA Polymerase,
pubmed-meshheading:9298217-Ribonuclease H
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pubmed:year |
1997
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pubmed:articleTitle |
Use of two reverse transcriptases eliminates false-positive results in differential display.
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pubmed:affiliation |
Columbia University College of Physicians and Surgeons New York, NY, USA.
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pubmed:publicationType |
Research Support, Non-U.S. Gov't,
Technical Report
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