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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
1997-10-2
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pubmed:abstractText |
The growth of U-87 or C6 gliomas co-implanted in nude mice with retroviral producer cells (VPC) expressing the herpes simplex virus-thymidine kinase (HSV-tk) gene is only partially impaired by treatment with ganciclovir (GCV). The effect of GCV is even less evident when C6 and VPC are co-implanted into the rat brain. Furthermore, tumors from C6 cells carrying the HSV-tk gene are not eradicated by GCV, although they remain sensitive to GCV when replated in vitro. These limits of the HSV-tk/GCV system in glioma gene therapy may be due to insufficient gene transfer and/or insufficient delivery of GCV to glioma cells. Combination of HSV-tk and one or more cytokines may improve the antitumor efficacy. Among cytokines, interleukin-4 (IL-4) has already been shown to be active against gliomas. In nude mice, GCV treatment inhibited tumor growth more effectively after co-injection of C6 cells with a mixture of VPC transducing IL-4 and HSV-tk genes than after co-injection with either IL-4 or HSV-tk VPC only. In immunocompetent Sprague-Dawley rats, co-injection of IL-4 VPC and C6 cells was also effective in inhibiting the growth of C6 brain tumors, 38% of the animals surviving for at least 2 months. Furthermore, increased and prolonged antitumor efficacy was obtained by transducing both IL-4 and HSV-tk genes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antimetabolites,
http://linkedlifedata.com/resource/pubmed/chemical/Ganciclovir,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1043-0342
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1345-53
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9295129-Animals,
pubmed-meshheading:9295129-Antimetabolites,
pubmed-meshheading:9295129-Female,
pubmed-meshheading:9295129-Ganciclovir,
pubmed-meshheading:9295129-Gene Therapy,
pubmed-meshheading:9295129-Gene Transfer Techniques,
pubmed-meshheading:9295129-Genetic Vectors,
pubmed-meshheading:9295129-Glioma,
pubmed-meshheading:9295129-Interleukin-4,
pubmed-meshheading:9295129-Mice,
pubmed-meshheading:9295129-Mice, Nude,
pubmed-meshheading:9295129-Rats,
pubmed-meshheading:9295129-Rats, Sprague-Dawley,
pubmed-meshheading:9295129-Retroviridae,
pubmed-meshheading:9295129-Simplexvirus,
pubmed-meshheading:9295129-Thymidine Kinase,
pubmed-meshheading:9295129-Transduction, Genetic,
pubmed-meshheading:9295129-Viral Proteins
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pubmed:year |
1997
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pubmed:articleTitle |
Limited efficacy of the HSV-TK/GCV system for gene therapy of malignant gliomas and perspectives for the combined transduction of the interleukin-4 gene.
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pubmed:affiliation |
Istituto Nazionale Neurologico C. Besta, Department of Biochemistry and Genetics, Milano, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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