Linked Life Data

9294262

Source:http://linkedlifedata.com/resource/pubmed/id/9294262

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-11-13
pubmed:databankReference
pubmed:abstractText
A DNA fragment containing an open reading frame of 2016 nucleotides has been cloned from the DNA of Aspergillus awamori by hybridization with a probe internal to the KAR2 (BiP) gene of Saccharomyces cerevisiae. The 73.4-kDa-encoded protein showed very high similarity to the endoplasmic reticulum (ER) lumenal BiP protein of S. cerevisiae, Kluyveromyces lactis, Schizosaccharomyces pombe, and animal and plant cells. The BiP protein contains a polar N-terminal end followed by a 18-amino-acid strongly hydrophobic region corresponding to the leader peptide for transport through the ER membrane. In the C-terminal region the protein ends with the HDEL canonical ER retention signal that targets proteins to the lumen of the ER. The A. awamori bip gene contains three introns as shown by cloning and sequencing the putative intron regions from a cDNA library. The bip gene is transcribed as a monocistronic mRNA of 2.4 kb. Two transcription start sites located 160 and 233 bp upstream of the first translated ATG were identified by primer extension. The promoter region showed no consensus TATA box but it contains CCAAT and CreA boxes known to be involved in both stress and carbon-catabolite regulation of fungal promoters.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0172-8083
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
139-46
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9294262-Amino Acid Sequence, pubmed-meshheading:9294262-Animals, pubmed-meshheading:9294262-Aspergillus, pubmed-meshheading:9294262-Base Sequence, pubmed-meshheading:9294262-Binding Sites, pubmed-meshheading:9294262-Carrier Proteins, pubmed-meshheading:9294262-Cloning, Molecular, pubmed-meshheading:9294262-Endoplasmic Reticulum, pubmed-meshheading:9294262-Evolution, Molecular, pubmed-meshheading:9294262-Fungal Proteins, pubmed-meshheading:9294262-HSP70 Heat-Shock Proteins, pubmed-meshheading:9294262-Heat-Shock Proteins, pubmed-meshheading:9294262-Introns, pubmed-meshheading:9294262-Mammals, pubmed-meshheading:9294262-Molecular Chaperones, pubmed-meshheading:9294262-Molecular Sequence Data, pubmed-meshheading:9294262-Oligopeptides, pubmed-meshheading:9294262-Open Reading Frames, pubmed-meshheading:9294262-Protein Sorting Signals, pubmed-meshheading:9294262-Sequence Homology, Amino Acid, pubmed-meshheading:9294262-Transcription, Genetic
pubmed:year
1997
pubmed:articleTitle
Characterization of the bip gene of Aspergillus awamori encoding a protein with an HDEL retention signal homologous to the mammalian BiP involved in polypeptide secretion.
pubmed:affiliation
Area of Microbiology, Faculty of Biology, University of León, 24071 León, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't