|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
1997-10-16
|
|
pubmed:abstractText |
Measles causes a profound immune suppression which is responsible for the high morbidity and mortality induced by secondary infections. Dendritic cells (DC) are professional antigen-presenting cells required for initiation of primary immune responses. To determine whether infection of DC by measles virus (MV) may play a role in virus-induced suppression of cell-mediated immunity, we examined the ability of CD1a+ DC derived from cord blood CD34+ progenitors and Langerhans cells isolated from human epidermis to support MV replication. Here we show that both cultured CD1a+ DC and epidermal Langerhans cells can be infected in vitro by both vaccine and wild type strains of MV. DC infection with MV resulted within 24-48 h in cell-cell fusion, cell surface expression of hemagglutinin, and virus budding associated with production of infectious virus. MV infection of DC completely abrogated the ability of the cells to stimulate the proliferation of naive allogeneic CD4+ T cell as early as day 2 of mixed leukocyte reaction (MLR) (i.e., on day 4 of DC infection). Mannose receptor-mediated endocytosis and viability studies indicated that the loss of DC stimulatory function could not be attributed to the death or apoptosis of DC. This total loss of DC stimulatory function required viral replication in the DC since ultraviolet (UV)-inactivated MV or UV-treated supernatant from MV-infected DC did not alter the allostimulatory capacity of DC. As few as 10 MV- infected DC could block the stimulatory function of 10(4) uninfected DC. More importantly, MV-infected DC, in which production of infectious virus was blocked by UV treatment or paraformaldehyde fixation, actively suppressed allogeneic MLR upon transfer to uninfected DC-T-cultures. Thus, the mechanisms which contribute to the loss of the allostimulatory function of DC include both virus release and active suppression mediated by MV-infected DC, independent of virus production. These data suggest that carriage of MV by DC may facilitate virus spreading to secondary lymphoid organs and that MV replication in DC may play a central role in the general immune suppression observed during measles.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-126271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-13801146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-14087040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-1696863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-1736530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-1910172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-1914259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-1993882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-2107264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-2145214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-2153236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-2414919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-2445889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-2476318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-2498661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-308511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-3132743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-3162253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-3601492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-3796316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-3877996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-4009115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-4201441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-4208453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-4605162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-5762650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-6230187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-6600445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-702064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-7594560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-7622868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-7629501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-7699319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-8040335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-8101549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-8145044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-8436173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-8600520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-8662504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-8666941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-8760823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-8917490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9294135-8917567
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0022-1007
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
186
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
801-12
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:9294135-CD4-Positive T-Lymphocytes,
pubmed-meshheading:9294135-Cell Communication,
pubmed-meshheading:9294135-Cell Survival,
pubmed-meshheading:9294135-Cells, Cultured,
pubmed-meshheading:9294135-Cytopathogenic Effect, Viral,
pubmed-meshheading:9294135-Dendritic Cells,
pubmed-meshheading:9294135-Hemagglutinins, Viral,
pubmed-meshheading:9294135-Humans,
pubmed-meshheading:9294135-Immune Tolerance,
pubmed-meshheading:9294135-Isoantigens,
pubmed-meshheading:9294135-Langerhans Cells,
pubmed-meshheading:9294135-Lymphocyte Activation,
pubmed-meshheading:9294135-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:9294135-Measles,
pubmed-meshheading:9294135-Measles virus,
pubmed-meshheading:9294135-Microscopy, Electron,
pubmed-meshheading:9294135-Virus Replication
|
|
pubmed:year |
1997
|
|
pubmed:articleTitle |
Measles virus infects human dendritic cells and blocks their allostimulatory properties for CD4+ T cells.
|
|
pubmed:affiliation |
Institut National de la Santé et de la Recherche Médicale U 404 "Immunité et Vaccination," Lyon, France.
|
|
pubmed:publicationType |
Journal Article
|
