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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1997-10-23
|
| pubmed:abstractText |
Retinoids inhibit proliferation of melanocytes and melanoma cells and affect disorders of hypo- and hyperpigmentation. Such effects might involve retinoid-binding proteins, retinoid metabolites and nuclear retinoid receptors for transcriptional activation. We detected messenger RNA transcripts for the cellular retinol- and retinoic acid-binding proteins (CRBP, CRABP I and II) in cultured epidermal melanocytes. In the melanoma cell lines the major transcript was CRABP II. Nuclear retinoic acid (RA) receptor transcripts and the 9-cis-retinoic acid receptor transcript were detected in all cells. The endogenous concentrations of retinol (ROH) and its metabolite 3,4-didehydroretinol (ddROH) in melanocytes were five times those in melanoma cells. When cells were incubated with [3H]ROH the main metabolites in the melanocytes were [3H]ddROH (4%) and [3H]RA (0.4%). Formation of [3H]RA was only detected in one melanoma cell line. Both melanocytes and melanoma cells produced an unidentified metabolite when incubated with [3H]ROH and [3H]RA. Dissimilarities in the metabolism and endogenous concentration of retinoids between benign and malignant melanocytes might play a key role in differentiation and growth regulation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Retinol-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Retinol-Binding Proteins, Cellular,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium,
http://linkedlifedata.com/resource/pubmed/chemical/Vitamin A,
http://linkedlifedata.com/resource/pubmed/chemical/retinol binding protein receptor
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0960-8931
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
267-74
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9293476-Cells, Cultured,
pubmed-meshheading:9293476-Child,
pubmed-meshheading:9293476-Gene Expression,
pubmed-meshheading:9293476-Humans,
pubmed-meshheading:9293476-Infant,
pubmed-meshheading:9293476-Infant, Newborn,
pubmed-meshheading:9293476-Male,
pubmed-meshheading:9293476-Melanocytes,
pubmed-meshheading:9293476-Melanoma,
pubmed-meshheading:9293476-Polymerase Chain Reaction,
pubmed-meshheading:9293476-RNA, Messenger,
pubmed-meshheading:9293476-Receptors, Cell Surface,
pubmed-meshheading:9293476-Retinol-Binding Proteins,
pubmed-meshheading:9293476-Retinol-Binding Proteins, Cellular,
pubmed-meshheading:9293476-Skin,
pubmed-meshheading:9293476-Tretinoin,
pubmed-meshheading:9293476-Tritium,
pubmed-meshheading:9293476-Tumor Cells, Cultured,
pubmed-meshheading:9293476-Vitamin A
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Vitamin A metabolism and mRNA expression of retinoid-binding protein and receptor genes in human epidermal melanocytes and melanoma cells.
|
| pubmed:affiliation |
Department of Dermatology, University Hospital, Linköping, Sweden.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|