Linked Life Data

9292349

Source:http://linkedlifedata.com/resource/pubmed/id/9292349

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1997-11-13
pubmed:abstractText
To gain information on the specific composition of the inflammatory infiltrate of genital ulcers caused by Haemophilus ducreyi, biopsies of 6 genital ulcers which were diagnosed as chancroid on clinical and microbiological grounds were subjected to immunohistochemical investigations after conventional haematoxylineosin staining. A variety of antibodies reactive against B- and T-cells, plasma cells and granulocytes were used with each tissue sections. The lymphocytic infiltrate of chancroid ulcers consisted of both B- and T-lymphocytes and showed a cluster-like formation. B-lymphocytes were preferentially localized perivascularly in the middle layer, T-lymphocytes mainly in the deep layer of the inflamed oedematous tissue. Results stress the importance of both B- and T-cell mediated immune responses in Haemophilus ducreyi infection.
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0956-4624
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
585-8
pubmed:dateRevised
2004-11-17
pubmed:otherAbstract
PIP: Chancroid, the most prevalent form of genital ulcer disease in developing countries, increases the risk of HIV transmission. Use of monoclonal antibodies against leukocyte differentiation antigens enabled analysis of the composition of the inflammatory infiltrate of genital ulcers. In this study, biopsies of six genital ulcers caused by Haemophilus ducreyi were examined immunohistochemically. In each case, staining revealed a superficial necrotic layer of polymorphonuclear leukocytes with fibrin and erythrocytes at the base of the ulcer, a middle layer of the edematous inflammatory dermis with prominent blood vessels and vascular thrombi, and a deep layer of an inflammatory infiltrate of plasma cells and lymphocytes. The lymphocytic infiltrate of chancroid ulcers consisted of both B- and T-lymphocytes and showed a cluster-like formation. B-lymphocytes were preferentially localized perivascularly in the middle layer, while T-lymphocytes tended to be located in the deep layer of the inflamed edematous tissue. These findings provide further evidence of the importance of the involvement of T-cells in the local immune clearance of H. ducreyi. Future studies should investigate lymphocyte secretions detected in genital ulcers caused by H. ducreyi to gain more information on the role of the cellular immune mechanisms in the disease.
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Immunohistochemical investigations of genital ulcers caused by Haemophilus ducreyi.
pubmed:affiliation
Department of Dermatology and Allergy, Technical University, Munich, Germany.
pubmed:publicationType
Journal Article