Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1997-10-1
|
| pubmed:abstractText |
We conducted this study to assess the clinical utility of heteroduplex analysis of polymerase chain reaction (PCR) products for Tgamma gene rearrangements in the diagnosis of T-cell lymphoproliferative disorders (LPDs) using a single set of consensus primers. This method was used to study 103 cases using fresh or frozen tissue. These included 62 cases of T-cell LPDs, 25 cases of B-cell LPDs, 3 cases of anaplastic large cell lymphomas of undefined lineage, and 13 cases of reactive disorders. The latter group served as the negative control group. For all cases of T-cell LPDs, the results of Tbeta Southern analysis (SA) and heteroduplex PCR analysis were compared. We also studied 10 cases of postthymic T-cell lymphoma using paraffin-embedded tissue. Heteroduplex Tgamma PCR was positive in 51 (89%) of 57 cases that were rearranged by Tbeta SA. Eight of the 10 cases of T-cell lymphoma analyzed using paraffin-embedded tissue were positive. There were no false-positive results. These results demonstrate that this method is an effective screening test in the clinical laboratory. A positive result obviates the need to perform SA. Preliminary results suggest this method is useful using paraffin-embedded tissue.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0002-9173
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
108
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
295-301
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9291458-Base Sequence,
pubmed-meshheading:9291458-Blotting, Southern,
pubmed-meshheading:9291458-DNA,
pubmed-meshheading:9291458-DNA Primers,
pubmed-meshheading:9291458-Flow Cytometry,
pubmed-meshheading:9291458-Gene Amplification,
pubmed-meshheading:9291458-Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor,
pubmed-meshheading:9291458-Humans,
pubmed-meshheading:9291458-Lymphoma, T-Cell,
pubmed-meshheading:9291458-Lymphoproliferative Disorders,
pubmed-meshheading:9291458-Nucleic Acid Heteroduplexes,
pubmed-meshheading:9291458-Pathology, Clinical,
pubmed-meshheading:9291458-Polymerase Chain Reaction,
pubmed-meshheading:9291458-T-Lymphocytes
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Clinical utility of heteroduplex analysis of TCR gamma gene rearrangements in the diagnosis of T-cell lymphoproliferative disorders.
|
| pubmed:affiliation |
Division of Laboratory Medicine, British Columbia Cancer Agency and the University of British Columbia, Vancouver, Canada.
|
| pubmed:publicationType |
Journal Article
|