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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1997-9-29
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pubmed:abstractText |
The biosynthesis of the furanic labdane diterpene marrubiin has been studied in plantlets and shoot cultures of Marrubium vulgare (Lamiaceae). The use of [2-14C]acetate, [2-14C]pyruvate, [2-14C]mevalonic acid and [U-14C]glucose incorporation experiments showed that the labelling of sterols in etiolated shoot cultures of M. vulgare was in accordance with their biosynthesis via the acetate-mevalonate pathway. In contrast, the incorporation rates of these precursors into the diterpene marrubiin could not be explained by biosynthesis of this compound via the acetate-mevalonate pathway. Cultivation of etiolated shoot cultures of M. vulgare on medium containing [1-13C]glucose and subsequent 13C-NMR spectroscopy of marrubiin led to the conclusion that the biosynthesis of marrubiin follows a non-mevalonate pathway. All isoprenic units of 13C-labelled marrubiin were enriched in those carbons that correspond to positions 1 and 5 of a putative precursor isopentenyl diphosphate. This labelling pattern from [1-13C]glucose is consistent with an alternative pathway via trioses, which has already been shown to occur in Eubacteria and Gymnospermae. The labdane skeleton is a precursor of many other skeletal types of diterpenes. Therefore it becomes obvious that in connection with the few known examples of a non-mevalonate pathway to isoprenoids the formation of some isoprenoids in plants via a non-mevalonate pathway might be quite common.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetates,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Isotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Diterpenes,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Mevalonic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Polyisoprenyl Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Pyruvates,
http://linkedlifedata.com/resource/pubmed/chemical/Terpenes,
http://linkedlifedata.com/resource/pubmed/chemical/geranylgeranyl pyrophosphate,
http://linkedlifedata.com/resource/pubmed/chemical/marrubiin
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
326 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
449-54
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9291117-Acetates,
pubmed-meshheading:9291117-Carbon Isotopes,
pubmed-meshheading:9291117-Carbon Radioisotopes,
pubmed-meshheading:9291117-Cells, Cultured,
pubmed-meshheading:9291117-Diterpenes,
pubmed-meshheading:9291117-Glucose,
pubmed-meshheading:9291117-Magnetic Resonance Spectroscopy,
pubmed-meshheading:9291117-Mevalonic Acid,
pubmed-meshheading:9291117-Plant Shoots,
pubmed-meshheading:9291117-Plants, Medicinal,
pubmed-meshheading:9291117-Polyisoprenyl Phosphates,
pubmed-meshheading:9291117-Pyruvates,
pubmed-meshheading:9291117-Terpenes,
pubmed-meshheading:9291117-Trees
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pubmed:year |
1997
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pubmed:articleTitle |
Biosynthesis of the labdane diterpene marrubiin in Marrubium vulgare via a non-mevalonate pathway.
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pubmed:affiliation |
Institute of Pharmaceutical Biology, University of Bonn, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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