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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1997-9-29
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/A49662,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/A61024,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Y11029,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z48234,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P00325,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P00326,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P00327,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P00328,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P00329,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P00333,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P04707,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P05336,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P06525,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P07327,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P10847,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P10848,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P12886,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P13603,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P14219,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P14673,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P14674,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P14675,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P17648,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P18332,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P19631,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P20306,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P23991,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P25141,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P28469,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P80222
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pubmed:abstractText |
We have previously shown that intact plants and cultured plant cells can metabolize and detoxify formaldehyde through the action of a glutathione-dependent formaldehyde dehydrogenase (FDH), followed by C-1 metabolism of the initial metabolite (formic acid). The cloning and heterologous expression of a cDNA for the glutathione-dependent formaldehyde dehydrogenase from Zea mays L. is now described. The functional expression of the maize cDNA in Escherichia coli proved that the cloned enzyme catalyses the NAD(+)- and glutathione (GSH)-dependent oxidation of formaldehyde. The deduced amino acid sequence of 41 kDa was on average 65% identical with class III alcohol dehydrogenase from animals and less than 60% identical with conventional plant alcohol dehydrogenases (ADH) utilizing ethanol. Genomic analysis suggested the existence of a single gene for this cDNA. Phylogenetic analysis supports the convergent evolution of ethanol-consuming ADHs in animals and plants from formaldehyde-detoxifying ancestors. The high structural conservation of present-day glutathione-dependent FDH in microorganisms, plants and animals is consistent with a universal importance of these detoxifying enzymes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0167-4412
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
843-54
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9290637-Alcohol Dehydrogenase,
pubmed-meshheading:9290637-Aldehyde Oxidoreductases,
pubmed-meshheading:9290637-Amino Acid Sequence,
pubmed-meshheading:9290637-Base Sequence,
pubmed-meshheading:9290637-Blotting, Northern,
pubmed-meshheading:9290637-Blotting, Southern,
pubmed-meshheading:9290637-Cloning, Molecular,
pubmed-meshheading:9290637-DNA, Complementary,
pubmed-meshheading:9290637-Escherichia coli,
pubmed-meshheading:9290637-Evolution, Molecular,
pubmed-meshheading:9290637-Formaldehyde,
pubmed-meshheading:9290637-Genes, Plant,
pubmed-meshheading:9290637-Glutathione,
pubmed-meshheading:9290637-Molecular Sequence Data,
pubmed-meshheading:9290637-NAD,
pubmed-meshheading:9290637-Polymerase Chain Reaction,
pubmed-meshheading:9290637-Seeds,
pubmed-meshheading:9290637-Sequence Analysis, DNA,
pubmed-meshheading:9290637-Sequence Homology, Amino Acid,
pubmed-meshheading:9290637-Tissue Distribution,
pubmed-meshheading:9290637-Zea mays
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pubmed:year |
1997
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pubmed:articleTitle |
Maize glutathione-dependent formaldehyde dehydrogenase cDNA: a novel plant gene of detoxification.
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pubmed:affiliation |
GSF-Forschungszentrum für Umwelt und Gesundheit, Institut für Biochemische Pflanzenpathologie, Oberschleissheim, Germany.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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