Linked Life Data

9289280

Source:http://linkedlifedata.com/resource/pubmed/id/9289280

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1997-9-8
pubmed:abstractText
The study was undertaken to study the specific features of transformation of E. coli strains having different R-chemotypes, Y. pestis, S. minnesota R595, and S. typhi Ty21a by plasmids carrying Yersinia pestis Fra-operon which controls the formation of a plague microbe capsular F1 antigen in this microorganism. Calcium transformation was shown to be rather effective for the plasmids constructed on the basis of a cosmid vector (pFS1), rather than those designed by using the Y. pestis plasmid pPst I (pFSK3, pP3). The level of plasmid stability varied and failed to correlate with taxonomy fitting and the chemotype of a recipient strain. The cells of all recombinant strains produced F1 antigen, secreted it into the environment; the synthesis was temperature-regulated. F1 was identified both in the diffuse precipitation and serological tests. The levels of F1 antigen synthesis decreased whereas nutritious requirements for the maintenance of protein synthesis increased for bacterial strains with higher levels of LPS reduction.
pubmed:language
rus
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0869-6047
pubmed:author
pubmed:issnType
Print
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
44-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
[Recombinant plasmids carrying yersinia pestis fra-operon: specific features of genetic transmission, inheritance and expression in attenuated enterobacterial cells].
pubmed:affiliation
State Scientific Center of Applied Microbiology, Obolensk.
pubmed:publicationType
Journal Article, English Abstract