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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1997-9-17
|
| pubmed:abstractText |
Through PCR amplifications of the gene encoding the merozoite surface antigen 2, utilizing allele-specific 3D7 and FC27 probes, we have examined the prevalence of Plasmodium falciparum in children aged from 7 to 14 years living in a village located in the equatorial forest region of Central Africa (Gabon). Using this technique, 61% (100/163) of the blood samples were shown to be infected with P. falciparum with 24 alleles distinguished by size polymorphism and sequence type. The two main families (3D7 and FC27) and hybrid alleles were detected regardless of sex and hemoglobin phenotype. No age-related changes in prevalence of P. falciparum strains were observed; however, the prevalence of infection (42%) was significantly lower in individuals with the sickle-cell trait compared with their normal-hemoglobin counterparts (68%). Mixtures of genetically distinct parasite clones were present in 82% of children carrying the sickle-cell trait but in only 58% of normal-hemoglobin carriers. The significance of these observations regarding the design and interpretation of epidemiological investigations is discussed in the context of malaria transmission in the region studied.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0014-4894
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
87
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
39-46
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9287956-Adolescent,
pubmed-meshheading:9287956-Alleles,
pubmed-meshheading:9287956-Animals,
pubmed-meshheading:9287956-Antigens, Protozoan,
pubmed-meshheading:9287956-Antigens, Surface,
pubmed-meshheading:9287956-Child,
pubmed-meshheading:9287956-Cohort Studies,
pubmed-meshheading:9287956-Gabon,
pubmed-meshheading:9287956-Genetic Variation,
pubmed-meshheading:9287956-Genotype,
pubmed-meshheading:9287956-Humans,
pubmed-meshheading:9287956-Malaria, Falciparum,
pubmed-meshheading:9287956-Plasmodium falciparum,
pubmed-meshheading:9287956-Polymerase Chain Reaction,
pubmed-meshheading:9287956-Polymorphism, Genetic,
pubmed-meshheading:9287956-Prevalence,
pubmed-meshheading:9287956-Protozoan Proteins,
pubmed-meshheading:9287956-Sickle Cell Trait
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Plasmodium falciparum: sickle-cell trait is associated with higher prevalence of multiple infections in Gabonese children with asymptomatic infections.
|
| pubmed:affiliation |
International Centre for Medical Research, Franceville, Gabon, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|