Linked Life Data

9285538

Source:http://linkedlifedata.com/resource/pubmed/id/9285538

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1997-10-9
pubmed:abstractText
Integrins alpha2beta1, alpha3beta1, and alpha6beta4 are expressed in the epidermis, and play an important role in wound healing and/or epidermal-dermal interaction. These integrins may provide a new perspective into the understanding of wound healing and vesication. The isolated perfused porcine skin flap (IPPSF) has been shown to be an in vitro model for chemical-induced vesication. In order to determine whether the IPPSF could be utilized to study skin diseases mediated by integrins, the expression of integrins alpha2beta1, alpha3beta1, and alpha6beta4 was studied in human skin, pig skin, and the IPPSF using immunohistochemical staining. Immunostaining of both alpha2beta1 and alpha3beta1 was primarily located at the periphery of the basal keratinocytes in human skin. Similarly, alpha2beta1 was expressed in the stratum basale layer of the epidermis in both pig skin and the IPPSF after 8 h of perfusion. These antibodies defined the periphery of the pig basal keratinocytes more diffusely than that of human cells. However, the alpha3 antibody outlined the keratinocytes in all epidermal layers of the IPPSF and in the pig skin. In human skin, pig skin, and the IPPSF, alpha6beta4 stained exclusively at the basal pole of the basal keratinocytes, and showed a continuous linear labeling along the epidermal-dermal junction. The IPPSF showed stronger immunoreactivity with the antibody against beta4. Furthermore, the distribution of alpha6beta4 in 5.0 mg/ml of bis-(2-chloroethyl) sulfide (sulfur mustard, HD)-induced blisters was examined in the IPPSF. The alpha6beta4 staining was exclusively located on the epidermal side (roof) of the blister. In addition, alpha6beta4 staining was not linear but disrupted and patchy. These findings suggest that any destruction of alpha6beta4 may weaken the epidermal-dermal junction, thereby leading to HD-induced vesication. This study demonstrates that the IPPSF expresses similar integrins to those of human skin, and the distribution of alpha6beta4 in the IPPSF blisters caused by HD is comparable to that of some human basement membrane blistering diseases. Therefore, the pig and the IPPSF prove to be ideal models to study the role of integrins in wound healing and blistering diseases occurring at the epidermal-dermal junction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0260-437X
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
247-53
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9285538-Animals, pubmed-meshheading:9285538-Antibodies, Monoclonal, pubmed-meshheading:9285538-Antigens, CD29, pubmed-meshheading:9285538-Antigens, Neoplasm, pubmed-meshheading:9285538-Antigens, Surface, pubmed-meshheading:9285538-Blister, pubmed-meshheading:9285538-Humans, pubmed-meshheading:9285538-Immunohistochemistry, pubmed-meshheading:9285538-Integrin alpha3beta1, pubmed-meshheading:9285538-Integrin alpha6beta4, pubmed-meshheading:9285538-Integrins, pubmed-meshheading:9285538-Mustard Gas, pubmed-meshheading:9285538-Organ Culture Techniques, pubmed-meshheading:9285538-Perfusion, pubmed-meshheading:9285538-Receptors, Collagen, pubmed-meshheading:9285538-Skin, pubmed-meshheading:9285538-Species Specificity, pubmed-meshheading:9285538-Swine, pubmed-meshheading:9285538-Wound Healing
pubmed:articleTitle
Comparison of integrins in human skin, pig skin, and perfused skin: an in vitro skin toxicology model.
pubmed:affiliation
Cutaneous Pharmacology and Toxicology Center, North Carolina State University, Raleigh 27606, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S.