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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1997-9-23
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pubmed:databankReference |
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pubmed:abstractText |
The addition of a poly-His C-terminal extension, designed to facilitate the purification of the protein, to the beta-lactamase of a thermophilic Bacillus licheniformis strain modified the site of action of the signal peptidase. This resulted in the secretion of a protein with a different N-terminus, showing that this type of protein engineering might not always be as 'neutral' as generally assumed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0014-5793
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pubmed:author |
pubmed-author:CharlierPP,
pubmed-author:DevreeseBB,
pubmed-author:DuezCC,
pubmed-author:FonzéEE,
pubmed-author:FrèreJ MJM,
pubmed-author:GuillaumeGG,
pubmed-author:Lamotte-BrasseurJJ,
pubmed-author:LedentPP,
pubmed-author:LejeuneAA,
pubmed-author:Rhazi-FilaliFF,
pubmed-author:SamynBB,
pubmed-author:ThammII,
pubmed-author:Van BeeumenJJ,
pubmed-author:VanhoveMM
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
413
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
194-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9280280-Bacillus,
pubmed-meshheading:9280280-Crystallography, X-Ray,
pubmed-meshheading:9280280-Histidine,
pubmed-meshheading:9280280-Kinetics,
pubmed-meshheading:9280280-Membrane Proteins,
pubmed-meshheading:9280280-Peptides,
pubmed-meshheading:9280280-Protein Folding,
pubmed-meshheading:9280280-Protein Processing, Post-Translational,
pubmed-meshheading:9280280-Protein Sorting Signals,
pubmed-meshheading:9280280-Recombinant Fusion Proteins,
pubmed-meshheading:9280280-Serine Endopeptidases,
pubmed-meshheading:9280280-beta-Lactamases
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pubmed:year |
1997
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pubmed:articleTitle |
Unexpected influence of a C-terminal-fused His-tag on the processing of an enzyme and on the kinetic and folding parameters.
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pubmed:affiliation |
Centre for Protein Engineering, Université de Liège, Institut de Chimie, Belgium.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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