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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1997-9-22
|
| pubmed:abstractText |
We have characterized a new human cell line AP-217, derived from the peripheral blood of a patient with chronic myeloid leukemia in blastic crisis. The analysis of cell surface antigens and ploidy showed that AP-217 was an erythro-megakaryocytic cell line. The effects of inducers of differentiation were studied and focused on retinoic acid (RA). Uninduced AP-217 cells produced a low level of hemoglobin (Hb) that showed a moderate but significant dose-dependent increase after 13 cis-RA induction (four times above the control at 10(-5) M). To outline this effect, AP-217 cells were cloned at limiting dilution. A subclone (clone 2) was isolated which expressed glycophorin A on 12% of cells, and showed a marked sensitivity to RA. After a 4 day induction with increasing concentrations of RA (1-10 x 10(-6) M) Hb production by clone 2 cells was enhanced 12 times over the control at the highest concentration (10(-5) M). No effect of RA on the Hb production of K-562 and HEL was observed. This increased Hb production occurred simultaneously with a growth inhibition in clonogenic cultures (20% reduction) associated with a drastic reduction of the colony size. Moreover, we demonstrated the expression of mRNA for the beta globin gene in clone 2 and AP-217-cells. This is the first report of a positive effect of RA on the erythroid differentiation of a human leukemic cell line.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0145-2126
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
21
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
529-37
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9279364-Adult,
pubmed-meshheading:9279364-Antigens, Surface,
pubmed-meshheading:9279364-Blotting, Northern,
pubmed-meshheading:9279364-Cell Differentiation,
pubmed-meshheading:9279364-Cell Division,
pubmed-meshheading:9279364-Cloning, Molecular,
pubmed-meshheading:9279364-Erythrocytes,
pubmed-meshheading:9279364-Globins,
pubmed-meshheading:9279364-Hemoglobins,
pubmed-meshheading:9279364-Humans,
pubmed-meshheading:9279364-Isotretinoin,
pubmed-meshheading:9279364-Karyotyping,
pubmed-meshheading:9279364-Leukemia, Myelogenous, Chronic, BCR-ABL Positive,
pubmed-meshheading:9279364-Male,
pubmed-meshheading:9279364-Megakaryocytes,
pubmed-meshheading:9279364-Polymerase Chain Reaction,
pubmed-meshheading:9279364-Tumor Cells, Cultured
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Effects of retinoic acid on a new human erythromegakaryocytic cell line AP-217.
|
| pubmed:affiliation |
Laboratoire d'Hématologie, CHU A.Michallon, Grenoble, France.
|
| pubmed:publicationType |
Journal Article
|