Linked Life Data

9278412

Source:http://linkedlifedata.com/resource/pubmed/id/9278412

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
36
pubmed:dateCreated
1997-10-2
pubmed:databankReference
pubmed:abstractText
To investigate the mechanism of nitrogen metabolite repression in the biotechnologically important fungus Penicillium chrysogenum a polymerase chain reaction approach was employed to identify transcription factors involved in this regulatory circuit, leading to the isolation of a new gene (nreB) encoding a 298 amino acid protein. Despite a low overall amino acid sequence identity of approximately 30%, it shares several features with Dal80p/Uga43p and Gzf3p/Nil2p, both repressors in nitrogen metabolism in Saccharomyces cerevisiae. All three proteins contain an N-terminal GATA-type zinc finger motif, displaying 86% amino acid sequence identity, and a putative leucine zipper motif in the C terminus. Northern blot analysis revealed the presence of two nreB transcripts, 1.8 and 1.5 kilobases in length, that differ in polyadenylation sites. The steady state level of both transcripts is subject to nitrogen metabolite repression. The putative DNA binding domain of NREB, expressed as a fusion protein in Escherichia coli, binds in vitro to GATA sites of its own 5'-upstream region as well as in the promoter of the nitrate assimilation gene cluster. Consistent with a role in the regulation of nitrogen metabolism, overexpression of nreB leads to repression of nitrate assimilatory genes. Hence, the simple view of nitrogen regulation by four GATA factors in yeast, but only one key regulator in filamentous ascomycetes seems no longer valid.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
272
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
22576-82
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9278412-Amino Acid Sequence, pubmed-meshheading:9278412-Bacterial Proteins, pubmed-meshheading:9278412-Base Sequence, pubmed-meshheading:9278412-Blotting, Northern, pubmed-meshheading:9278412-Cloning, Molecular, pubmed-meshheading:9278412-DNA, Recombinant, pubmed-meshheading:9278412-Escherichia coli, pubmed-meshheading:9278412-GATA Transcription Factors, pubmed-meshheading:9278412-Genes, Fungal, pubmed-meshheading:9278412-Molecular Sequence Data, pubmed-meshheading:9278412-Multigene Family, pubmed-meshheading:9278412-Nitrates, pubmed-meshheading:9278412-Penicillium chrysogenum, pubmed-meshheading:9278412-Promoter Regions, Genetic, pubmed-meshheading:9278412-RNA, Messenger, pubmed-meshheading:9278412-Repressor Proteins, pubmed-meshheading:9278412-Sequence Homology, Amino Acid
pubmed:year
1997
pubmed:articleTitle
Overexpression of nreB, a new GATA factor-encoding gene of Penicillium chrysogenum, leads to repression of the nitrate assimilatory gene cluster.
pubmed:affiliation
Department of Microbiology (Medical School), University of Innsbruck, Fritz-Pregl Str. 3, A-6020 Innsbruck, Austria. hubertus.haas@uibk.ac.at
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't