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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1997-9-19
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pubmed:abstractText |
Components of the extracellular matrix (ECM) can regulate leukocyte activation and function at inflammatory sites. Low molecular weight fragments of the ECM glycosaminoglycan hyaluronan (LMW-HA) that accumulate in inflammation, but not the ubiquitous high molecular weight form of HA (HMW-HA), have been shown to induce cytokine and/or chemokine production by alveolar and bone-marrow derived macrophages. To determine the cellular requirements for responsiveness to HA, we compared the effects of HMW-HA and LMW-HA on resident and thioglycollate-elicited murine peritoneal macrophages. We demonstrate that treatment of elicited macrophages with LMW-HA, but not with HMW-HA, stimulated production of the chemokines RANTES and macrophage inflammatory protein-1alpha and -1beta. Further, we demonstrate that LMW-HA induced the production of biologically active IL-12, a proinflammatory cytokine not previously known to be regulated by cell-matrix interactions. The LMW-HA-induced production of IL-12 by elicited macrophages was inhibited by an anti-CD44 mAb that blocks HA binding. In contrast to elicited macrophages, freshly explanted resident peritoneal macrophages did not respond to LMW-HA. However, preculture in vitro before stimulation led to adhesion-dependent priming for LMW-HA-induced cytokine and chemokine production by resident macrophages. These results provide further evidence of the potential importance of CD44/LMW-HA interactions in regulating the immune response at sites of inflammation and demonstrate that the state of differentiation of macrophages may determine their sensitivities to matrix components.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD44,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines,
http://linkedlifedata.com/resource/pubmed/chemical/Hyaluronic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Thioglycolates
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
159
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2492-500
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9278343-Animals,
pubmed-meshheading:9278343-Antibodies, Monoclonal,
pubmed-meshheading:9278343-Antigens, CD44,
pubmed-meshheading:9278343-Cell Adhesion,
pubmed-meshheading:9278343-Chemokines,
pubmed-meshheading:9278343-Extracellular Matrix,
pubmed-meshheading:9278343-Female,
pubmed-meshheading:9278343-Gene Expression Regulation,
pubmed-meshheading:9278343-Hyaluronic Acid,
pubmed-meshheading:9278343-Inflammation,
pubmed-meshheading:9278343-Interferon-gamma,
pubmed-meshheading:9278343-Interleukin-12,
pubmed-meshheading:9278343-Macrophage Activation,
pubmed-meshheading:9278343-Macrophages, Peritoneal,
pubmed-meshheading:9278343-Mice,
pubmed-meshheading:9278343-Mice, Inbred C3H,
pubmed-meshheading:9278343-Molecular Weight,
pubmed-meshheading:9278343-Peritoneum,
pubmed-meshheading:9278343-Peritonitis,
pubmed-meshheading:9278343-Thioglycolates
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pubmed:year |
1997
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pubmed:articleTitle |
Induction of IL-12 and chemokines by hyaluronan requires adhesion-dependent priming of resident but not elicited macrophages.
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pubmed:affiliation |
Immunology Graduate Group, University of Pennsylvania, Philadelphia 19104, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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