Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1997-9-19
pubmed:abstractText
Components of the extracellular matrix (ECM) can regulate leukocyte activation and function at inflammatory sites. Low molecular weight fragments of the ECM glycosaminoglycan hyaluronan (LMW-HA) that accumulate in inflammation, but not the ubiquitous high molecular weight form of HA (HMW-HA), have been shown to induce cytokine and/or chemokine production by alveolar and bone-marrow derived macrophages. To determine the cellular requirements for responsiveness to HA, we compared the effects of HMW-HA and LMW-HA on resident and thioglycollate-elicited murine peritoneal macrophages. We demonstrate that treatment of elicited macrophages with LMW-HA, but not with HMW-HA, stimulated production of the chemokines RANTES and macrophage inflammatory protein-1alpha and -1beta. Further, we demonstrate that LMW-HA induced the production of biologically active IL-12, a proinflammatory cytokine not previously known to be regulated by cell-matrix interactions. The LMW-HA-induced production of IL-12 by elicited macrophages was inhibited by an anti-CD44 mAb that blocks HA binding. In contrast to elicited macrophages, freshly explanted resident peritoneal macrophages did not respond to LMW-HA. However, preculture in vitro before stimulation led to adhesion-dependent priming for LMW-HA-induced cytokine and chemokine production by resident macrophages. These results provide further evidence of the potential importance of CD44/LMW-HA interactions in regulating the immune response at sites of inflammation and demonstrate that the state of differentiation of macrophages may determine their sensitivities to matrix components.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
159
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2492-500
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9278343-Animals, pubmed-meshheading:9278343-Antibodies, Monoclonal, pubmed-meshheading:9278343-Antigens, CD44, pubmed-meshheading:9278343-Cell Adhesion, pubmed-meshheading:9278343-Chemokines, pubmed-meshheading:9278343-Extracellular Matrix, pubmed-meshheading:9278343-Female, pubmed-meshheading:9278343-Gene Expression Regulation, pubmed-meshheading:9278343-Hyaluronic Acid, pubmed-meshheading:9278343-Inflammation, pubmed-meshheading:9278343-Interferon-gamma, pubmed-meshheading:9278343-Interleukin-12, pubmed-meshheading:9278343-Macrophage Activation, pubmed-meshheading:9278343-Macrophages, Peritoneal, pubmed-meshheading:9278343-Mice, pubmed-meshheading:9278343-Mice, Inbred C3H, pubmed-meshheading:9278343-Molecular Weight, pubmed-meshheading:9278343-Peritoneum, pubmed-meshheading:9278343-Peritonitis, pubmed-meshheading:9278343-Thioglycolates
pubmed:year
1997
pubmed:articleTitle
Induction of IL-12 and chemokines by hyaluronan requires adhesion-dependent priming of resident but not elicited macrophages.
pubmed:affiliation
Immunology Graduate Group, University of Pennsylvania, Philadelphia 19104, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't