Linked Life Data

9278228

Source:http://linkedlifedata.com/resource/pubmed/id/9278228

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1997-10-14
pubmed:abstractText
To circumvent limitations imposed by conventional gene transfer techniques into cardiac muscle cells, we studied whether replication defective adenovirus would obviate this limitation to basic studies of signal transduction and transcriptional control processes in the heart. We demonstrate here the utility of adenovirus mediated gene transfer to introduce foreign DNA into post-mitotic terminally differentiated ventricular myocytes with uniformity and high efficiency. We also provide evidence for the genetic modification of neonatal ventricular myocytes by adenovirus early region 1 (E1) proteins and their impact on cardiac gene transcription and DNA synthesis respectively. Thus, for studies of transcriptional control processes in the heart, which until now have been restricted to neonatal ventricular myocytes; adenovirus mediated gene transfer provides a means to genetically manipulate adult cardiac muscle cells. The advent of adenovirus gene transfer will extend our understanding of the molecular mechanisms that mediate basic cardiac disease and may ultimately provide a means to therapeutically mitigate the disease process.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0300-8177
pubmed:author
pubmed:issnType
Print
pubmed:volume
172
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
13-21
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Adenovirus mediated-gene transfer into cardiomyocytes.
pubmed:affiliation
Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, University of Manitoba, Winnipeg, Canada.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't