Linked Life Data

9277347

Source:http://linkedlifedata.com/resource/pubmed/id/9277347

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2 Pt 1
pubmed:dateCreated
1997-9-24
pubmed:abstractText
Muscarinic stimulation of fura 2-loaded smooth muscle cells evoked a rapidly inactivating Ca(2+)-activated Cl- current [ICl(Ca)] and a sustained nonselective cation current (Icat) as well as a transient (delta Ca(tran)) and a sustained (delta Ca(sus)) elevation of cytosolic Ca2+ concentration ([Ca2+]i). Caffeine and inositol 1,4,5-trisphosphate induced delta Ca(tran) and ICl(Ca) but not Icat or delta Ca(sus). M2 receptor antagonism blocked muscarinic activation of Icat and delta Ca(sus) but not ICl(Ca) and delta Ca(tran). M3 antagonism blocked activation of ICl(Ca) and Icat and a rise in [Ca2+]i, but application of caffeine with methacholine restored Icat and delta Ca(sus). After depletion of intracellular Ca2+ stores, methacholine failed to induce Icat or a [Ca2+]i increase and, in pertussis toxin-treated cells, ICl(Ca) and delta Ca(tran) but not Icat or delta Ca(sus) were evoked. Anti-G alpha i-1/G alpha i-2 antibodies and anti-G alpha i-3/ G(o) alpha antibodies blocked Icat but did not affect ICl(Ca). Anti-Gq alpha/ G alpha 11 antibodies greatly inhibited ICl(Ca) but did not affect Icat. Activation of M2 receptors leads to the opening of nonselective cation channels through Gi/G(o) proteins in smooth muscle cells, resulting in a sustained rise in [Ca2+]i. Arise in [Ca2+]i is necessary but not sufficient for activation of nonselective cation channels.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
273
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C500-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
M2 receptor activation of nonselective cation channels in smooth muscle cells: calcium and Gi/G(o) requirements.
pubmed:affiliation
Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104-6046, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.