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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1997-9-9
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pubmed:abstractText |
TAO is characterized by an autoimmune process affecting the orbital contents. T cells have been suggested to have a major role in pathogenesis, but so far only limited data are available to clarify the extraocular muscle (EOM)-infiltrating T cell phenotype, antigenic reactivity and cytokine profile in TAO patients. In the present study, biopsies of affected EOM were taken and the infiltrating T cells isolated and expanded in vitro with mitogen. Their phenotype was determined by flow cytometric (FACS) analysis and compared with peripheral blood-derived T cell lines, treated in the same way from the same patient. Cytokines present in the supernatant after mitogen stimulation of the T cell lines were assayed by ELISA. In addition, cytokine mRNA present at the time of biopsy was determined by rapid RNA extraction from EOM and reverse transcription-amplification with specific cytokine oligonucleotide probes (IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-13, IL- 15, interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha)). In the T cell lines from two patients, proliferation assays were carried out with antigens derived from thyroid gland, EOM and a thyrotropin (TSH) receptor preparation. Most T cell lines were CD4+, CD45RO+, and TCR alpha/beta+, both from the EOM and the peripheral blood. A wide variety of cytokines was detected by analysis of supernatants or mRNA, but the profiles were not identical comparing the two approaches. However, IL-4 was detected by both. Dose-dependent proliferation was observed in response to thyroid extract in a biopsy-derived T cell line. In conclusion, EOM-infiltrating T cells from patients with TAO, expanded in vitro, were chiefly CD4+ and produced a mixture of cytokines, including IL-4. The proliferation data suggest that there are thyroid-reactive T cells in EOM.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0009-9104
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
109
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
362-9
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pubmed:dateRevised |
2010-8-25
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pubmed:meshHeading |
pubmed-meshheading:9276534-Adult,
pubmed-meshheading:9276534-Aged,
pubmed-meshheading:9276534-Antigens, CD,
pubmed-meshheading:9276534-Cytokines,
pubmed-meshheading:9276534-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:9276534-Female,
pubmed-meshheading:9276534-Flow Cytometry,
pubmed-meshheading:9276534-Graves Disease,
pubmed-meshheading:9276534-Humans,
pubmed-meshheading:9276534-Immunophenotyping,
pubmed-meshheading:9276534-Male,
pubmed-meshheading:9276534-Middle Aged,
pubmed-meshheading:9276534-Oculomotor Muscles,
pubmed-meshheading:9276534-RNA, Messenger,
pubmed-meshheading:9276534-T-Lymphocytes
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pubmed:year |
1997
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pubmed:articleTitle |
Analysis of extraocular muscle-infiltrating T cells in thyroid-associated ophthalmopathy (TAO).
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pubmed:affiliation |
Moorfields Eye Hospital NHS Trust and Department of Clinical Ophthalmology, Institute of Ophthalmology, London, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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