Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0004799,
umls-concept:C0007589,
umls-concept:C0031911,
umls-concept:C0181078,
umls-concept:C0205199,
umls-concept:C0221920,
umls-concept:C0221928,
umls-concept:C0243071,
umls-concept:C0332282,
umls-concept:C0332835,
umls-concept:C1511938,
umls-concept:C1516920,
umls-concept:C1522492,
umls-concept:C1705241,
umls-concept:C1705242
|
| pubmed:issue |
3
|
| pubmed:dateCreated |
1997-9-11
|
| pubmed:abstractText |
This study evaluated the in vitro and in vivo function of composite skin equivalents based on two different dermal analogs. Keratinocytes derived from the same dark-skinned neonatal foreskins were seeded onto both acellular human dermis and fibroblast-contracted collagen gels. Each type of composite graft readily formed an epithelium in vitro. However, the undulating surface of the acellular dermis acted as a template and organized the seeded keratinocytes into a rete ridge-like pattern, whereas the smooth surface of the fibroblast-contracted collagen gels generated an epithelium with a linear basal layer. Moreover, when acellular dermis was used, the composite grafts demonstrated enhanced melanocyte proliferation. When transplanted to athymic mice, both composite grafts formed a fully differentiated human epidermis, but repigmentation of the grafts when acellular dermis was used was more extensive and only the epidermis on the fibroblast-contracted collagen gels showed signs of hyperproliferation at 6 weeks after grafting. These results demonstrate that the type of dermal analog incorporated into a composite skin graft can influence the subsequent functionality of the skin substitute.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0041-1337
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
64
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
454-65
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9275113-Animals,
pubmed-meshheading:9275113-Basement Membrane,
pubmed-meshheading:9275113-Cell Differentiation,
pubmed-meshheading:9275113-Cells, Cultured,
pubmed-meshheading:9275113-Collagen,
pubmed-meshheading:9275113-Epidermis,
pubmed-meshheading:9275113-Epithelial Cells,
pubmed-meshheading:9275113-Gels,
pubmed-meshheading:9275113-Humans,
pubmed-meshheading:9275113-Immunohistochemistry,
pubmed-meshheading:9275113-Infant, Newborn,
pubmed-meshheading:9275113-Keratinocytes,
pubmed-meshheading:9275113-Keratins,
pubmed-meshheading:9275113-Male,
pubmed-meshheading:9275113-Melanocytes,
pubmed-meshheading:9275113-Mice,
pubmed-meshheading:9275113-Mice, Nude,
pubmed-meshheading:9275113-Skin,
pubmed-meshheading:9275113-Skin Pigmentation,
pubmed-meshheading:9275113-Skin Transplantation,
pubmed-meshheading:9275113-Staining and Labeling
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Differences in dermal analogs influence subsequent pigmentation, epidermal differentiation, basement membrane, and rete ridge formation of transplanted composite skin grafts.
|
| pubmed:affiliation |
Surgical Services, Massachusetts General Hospital, and the Shriners Burns Institute, Boston 02114, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|