Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1997-9-19
|
| pubmed:abstractText |
Tyrosine is a precursor of melanin synthesis and might thus present a valuable marker for melanoma. The aim of this study was to evaluate the uptake of alpha-methyl-tyrosine (AMT) in melanoma cell cultures and to assess its usefulness as a radiopharmaceutical for staging melanoma patients with whole-body scintigraphy. Melanoma (M19-cell lines) and fibroblast (negative control) cell cultures were incubated with 125I-AMT and the radioactive uptake in the cell lines was measured in a gamma-counter over 24 h. For in vivo studies, planar whole-body scintigraphy and single photon emission computed tomography (SPECT) of the tumour region was performed following injection of 250-350 MBq 123I-AMT in six patients with known melanoma metastases. Findings were compared with results of whole-body positron emission tomography using 18F-fluorodeoxyglucose (FDG-PET) as a standard of reference. Fibroblasts showed an unchanged uptake of (mean +/- SD) 0.56 +/- 0.09% 15 min and 0.066 +/- 0.09% 24 h, respectively, after incubation of 125I-AMT, whereas there was an increased uptake in melanoma cell cultures over time from 0.9 +/- 0.05% to 7.5 +/- 1.6%. In staging melanoma patients, the sensitivity of whole-body AMT-scintigraphy compared with FDG-PET was 37% (10 of 27 metastases). AMT is transported and metabolized to a high extent in melanoma cells and 123I-AMT is accumulated in melanoma metastases. Owing to its low sensitivity, however, the clinical use of whole-body AMT scintigraphy cannot be recommended.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Methyltyrosines,
http://linkedlifedata.com/resource/pubmed/chemical/Radiopharmaceuticals,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-Methyltyrosine
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0007-0963
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
137
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
96-100
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9274632-Adult,
pubmed-meshheading:9274632-Aged,
pubmed-meshheading:9274632-Cells, Cultured,
pubmed-meshheading:9274632-Enzyme Inhibitors,
pubmed-meshheading:9274632-Female,
pubmed-meshheading:9274632-Fibroblasts,
pubmed-meshheading:9274632-Humans,
pubmed-meshheading:9274632-Iodine Radioisotopes,
pubmed-meshheading:9274632-Male,
pubmed-meshheading:9274632-Melanoma,
pubmed-meshheading:9274632-Methyltyrosines,
pubmed-meshheading:9274632-Middle Aged,
pubmed-meshheading:9274632-Neoplasm Staging,
pubmed-meshheading:9274632-Radiopharmaceuticals,
pubmed-meshheading:9274632-Sensitivity and Specificity,
pubmed-meshheading:9274632-Skin Neoplasms,
pubmed-meshheading:9274632-Tomography, Emission-Computed,
pubmed-meshheading:9274632-Tomography, Emission-Computed, Single-Photon,
pubmed-meshheading:9274632-alpha-Methyltyrosine
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Radioiodine-labelled alpha-methyl-tyrosine in malignant melanoma: cell culture studies and results in patients.
|
| pubmed:affiliation |
Department of Dermatology, University Hospital of Zurich, Switzerland.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|