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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1997-9-29
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pubmed:abstractText |
The present study examined the effects of 0.1% dimethyl sulfoxide (DMSO) on nicotinic acetylcholine receptors (nAChR) from mouse muscle and Torpedo californica electrocytes. Receptors were expressed in Xenopus laevis oocytes and studied with voltage-clamp. When applied simultaneously with acetylcholine, DMSO did not inhibit current amplitude of either receptor. Preincubation with DMSO for 1 min reduced current amplitude by approximately 50% from oocytes expressing electrocyte receptor. Preincubation did not affect the muscle receptor. With electric organ membranes, 0.1% DMSO did not block either [alpha-(125)I]bungarotoxin binding to the nAChR agonist site or [3H]phencyclidine binding to its high affinity site on resting or desensitized receptor. These data suggest that DMSO might be affecting the electrocyte receptor through a second messenger system.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0304-3940
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
230
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
163-6
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9272686-Administration, Topical,
pubmed-meshheading:9272686-Animals,
pubmed-meshheading:9272686-Anti-Inflammatory Agents,
pubmed-meshheading:9272686-Bungarotoxins,
pubmed-meshheading:9272686-Dimethyl Sulfoxide,
pubmed-meshheading:9272686-Excitatory Amino Acid Antagonists,
pubmed-meshheading:9272686-Female,
pubmed-meshheading:9272686-Iodine Radioisotopes,
pubmed-meshheading:9272686-Ion Channel Gating,
pubmed-meshheading:9272686-Mice,
pubmed-meshheading:9272686-Oocytes,
pubmed-meshheading:9272686-Patch-Clamp Techniques,
pubmed-meshheading:9272686-Phencyclidine,
pubmed-meshheading:9272686-Radioligand Assay,
pubmed-meshheading:9272686-Receptors, Nicotinic,
pubmed-meshheading:9272686-Torpedo,
pubmed-meshheading:9272686-Tritium,
pubmed-meshheading:9272686-Xenopus laevis
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pubmed:year |
1997
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pubmed:articleTitle |
Differential effects of dimethyl sulfoxide on nicotinic acetylcholine receptors from mouse muscle and Torpedo electrocytes.
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pubmed:affiliation |
Department of Biochemistry and Nutrition, Center for Molecular and Behavioral Neuroscience, Universidad Central del Caribe, Bayamón, Puerto Rico. eaton@coqui.net
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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