Linked Life Data

9271342

Source:http://linkedlifedata.com/resource/pubmed/id/9271342

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-9-5
pubmed:abstractText
Western blot analysis was used to determine the concentration of the aryl hydrocarbon receptor nuclear translocator (ARNT) protein and aryl hydrocarbon receptor (AHR) in 11 mammalian cell culture lines derived from hepatic and nonhepatic tissues. The strategy was to first use Western blot analysis to determine the expression of ARNT or AHR in each cell line relative to its concentration in murine wild-type Hepa-1c1c7 (Hepa-1) cells. Actual ARNT and AHR concentrations in known amounts of total cell lysates were then determined by generating a standard curve with defined amounts of a highly purified ARNT or AHR protein and performing regression analysis. The results show that the level of ARNT expression in each of the cell lines is similar and represents approximately 0.001-0.002% of total cellular protein. The range of expression was only approximately 3-fold with wild-type Hepa-1 cells expressing the highest level of ARNT (33,000/cell) and canine kidney cells (MDCK line) expressing 14,000 ARNT molecules/cell. In contrast, the concentration of AHR varied by 65-fold over the different cell lines with the wild-type Hepa-1 expressing 323,000 AHR/cell and rat hepatoma cells (H4IIE) expressing 4700. The ratio of AHR to ARNT ranged from 0.3 in H4IIE cells to 10 in the Hepa-1 line with the majority of cells expressing 1-5 times more AHR than ARNT protein. Immunocytochemical staining of each cell line showed that ARNT was exclusively localized to the nuclear compartment and that a conserved nuclear localization signal mapped to the NH-terminal portion of the protein.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0026-895X
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
202-11
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9271342-Amino Acid Sequence, pubmed-meshheading:9271342-Animals, pubmed-meshheading:9271342-Aryl Hydrocarbon Receptor Nuclear Translocator, pubmed-meshheading:9271342-Blotting, Western, pubmed-meshheading:9271342-Cell Compartmentation, pubmed-meshheading:9271342-Cell Line, pubmed-meshheading:9271342-DNA-Binding Proteins, pubmed-meshheading:9271342-Dogs, pubmed-meshheading:9271342-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:9271342-Humans, pubmed-meshheading:9271342-Immunologic Techniques, pubmed-meshheading:9271342-Liver, pubmed-meshheading:9271342-Mice, pubmed-meshheading:9271342-Molecular Sequence Data, pubmed-meshheading:9271342-Molecular Weight, pubmed-meshheading:9271342-Nuclear Proteins, pubmed-meshheading:9271342-Rats, pubmed-meshheading:9271342-Receptors, Aryl Hydrocarbon, pubmed-meshheading:9271342-Transcription Factors
pubmed:year
1997
pubmed:articleTitle
Determination of aryl hydrocarbon receptor nuclear translocator protein concentration and subcellular localization in hepatic and nonhepatic cell culture lines: development of quantitative Western blotting protocols for calculation of aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator protein in total cell lysates.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, 29425, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't