|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
8
|
|
pubmed:dateCreated |
1997-9-24
|
|
pubmed:abstractText |
To investigate the role of the cellular ras gene product in neoplastic transformation by the SV40 large tumor antigen (SVLT), murine C3H10T1/2 cells were rendered deficient in Ras activity by transfection with inducible or constitutive antisense ras gene constructs or through the introduction of the dominant-negative mutant, ras(asn17). Consistent with previous results, SVLT-induced morphological transformation was unaffected by the down-regulation of c-ras gene product activity. On the other hand, colony formation in soft agar and tumorigenicity in nude mice were drastically reduced in c-Ras-deficient cells. In addition, SVLT expression in C3H10T1/2 cells led to increased c-Ras activity, as determined by an increase in the Ras-bound GTP/GTP + GDP ratio. These results suggest that c-Ras is required for full neoplastic transformation by SVLT.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
1044-9523
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
8
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
891-901
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:9269898-Animals,
pubmed-meshheading:9269898-Antigens, Polyomavirus Transforming,
pubmed-meshheading:9269898-Cell Line, Transformed,
pubmed-meshheading:9269898-Cell Transformation, Neoplastic,
pubmed-meshheading:9269898-Fibroblasts,
pubmed-meshheading:9269898-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:9269898-Genes, ras,
pubmed-meshheading:9269898-Guanosine Diphosphate,
pubmed-meshheading:9269898-Guanosine Triphosphate,
pubmed-meshheading:9269898-Mice,
pubmed-meshheading:9269898-Mice, Nude,
pubmed-meshheading:9269898-Mutation,
pubmed-meshheading:9269898-Neoplasms, Experimental,
pubmed-meshheading:9269898-Oncogene Proteins v-raf,
pubmed-meshheading:9269898-RNA, Antisense,
pubmed-meshheading:9269898-Retroviridae Proteins, Oncogenic,
pubmed-meshheading:9269898-Simian virus 40,
pubmed-meshheading:9269898-Transfection,
pubmed-meshheading:9269898-ras Proteins
|
|
pubmed:year |
1997
|
|
pubmed:articleTitle |
Cellular ras gene activity is required for full neoplastic transformation by the large tumor antigen of SV40.
|
|
pubmed:affiliation |
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada. raptisl@post.queensu.ca
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
